RAPIDATA AND OTHER THINGS

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 国际上最广泛认可的大分子晶体学方法课程之一在国家同步加速器光源（NSLS）举行。自1999年以来，来自世界各地的学生每年春天都会聚集在NSLS参加RapiData，这是一个为期一周的速成班，由Sweet，Alex Soares和Sal Sclafani组织，是P41项目的培训部分。它的目的是向参与者介绍最好的和最新的设备和技术的大分子x射线晶体学。RapiData允许学生与领先的软件开发人员会面并向他们学习 在晶体学领域，然后实际使用NSLS光束线来收集数据。今年，52名学生参加了4月4日至9日举行的工作坊。 自创建以来，已有近650名学生参加了这门课程，我们不断要求增加更多的插槽。许多RapiData校友现在已经成长为晶体学专家，他们继续将经验不足的同事推荐给BNL，以了解这个高度专业化领域的初始步骤。我们经常让前RapiData学生的研究生。 该课程开始于Sweet教授的为期一天的背景课程，旨在为经验不足的学生更新晶体学基础知识。接下来的两天充满了来自BNL，工业界，学术界和其他国家实验室的科学家的讲座和辅导。接下来，学生们被分成几组，并在八条NSLS光束线上进行了一场马拉松、60小时的数据收集会议。与此同时，许多教程正在进行中，由讲师自己运行。近三分之二的学生带来了自己的标本，目的是解决特定酶的结构，而其他人则观察和帮助。在这个过程中，大约有六个结构被解决了，每个结构都可能是一个可验证的结果。在课程的数据收集部分，学生们每天下午聚集在一起讨论他们的结果，并制作关于他们的数据和科学主题的迷你讲座。 看到学生们在一周内的技能发展总是令人兴奋的。他们中的许多人来这里时几乎没有晶体学经验，他们离开时知道如何获得和处理真实的数据，以及如何在出现问题时定位和解决问题。这就是有这样一个动手程序的好处。 这笔赠款用于培训班本身，BER/DOE提供实物资助，并为当地工作人员和光束线操作提供部分支助。布鲁克海文科学协会、NSLS和几家设备供应商和制药公司提供了额外的支持。此外，国际晶体学联合会还提供了一笔特别赠款，协助7名拉丁美洲学生参加培训班。 此外，当然，获取和处理良好衍射数据所需的技能培训是一个持续的过程，因为我们的科学家和PX操作员与我们光束线的外部用户互动。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. One of the most widely recognized courses internationally in macromolecular crystallography methods is held at the National Synchrotron Light Source (NSLS). Since 1999, students from around the world have gathered at the NSLS every spring for RapiData, a weeklong crash course organized by Sweet, Alex Soares, and Sal Sclafani, a training component of this P41 project. It's designed to introduce participants to the best and latest equipment and techniques for macromolecular x-ray crystallography. RapiData allows students to meet and learn from the leading developers of software in the crystallography field, and then to actually use NSLS beamlines to collect data. This year, 52 students attended the workshop, which was held from April 4 - 9. Since its creation, nearly 650 students have attended this course, and we have constant requests to add more slots. Many RapiData alumni have now blossomed into crystallography experts, and they continue to refer their less-experienced colleagues to BNL to learn the initial steps of this highly specialized area of interest. We regularly get graduate students of former RapiData students. The course began with a daylong background session taught by Sweet, meant to refresh the knowledge of crystallography fundamentals for less-experienced students. The following two days were filled with lectures and tutorials taught by scientists from BNL, industry, academia, and other national labs. Next, the students were divided into groups and guided through a marathon, 60-hour data-collection session on eight NSLS beamlines. At the same time, many tutorials were underway, run by the lecturers themselves. Nearly two-thirds of the students brought their own specimens with the goal of solving the structure of a particular enzyme, while the others observed and helped. About half a dozen structures were solved during the course, each of which could be a publishable result. During the data-collection portion of the course, the students gathered each afternoon to discuss their results and produced mini lectures about their data and scientific subject. It's always really exciting to watch the students' skills develop throughout the week. Many of them come here with little or no experience in crystallography, and they leave knowing how to obtain and process real data, as well as how to locate and fix problems as they arise. That's the advantage of having such a hands-on program. There was support from this grant for the course itself, and BER/DOE provided in-kind funding with their partial support of local staff and beamline operations. Additional support was provided by Brookhaven Science Associates, the NSLS, and several equipment vendors and drug companies. In addition a special grant was provided by the International Union of Crystallography to assist seven Latin American students in attending the course. In addition, of course, training in the skills required to take and process good diffraction data are a continuous process as our scientists and PX Operators interact with outside users of our beamlines.