NMR OF ONE GLYCOLIPID SAMPLE

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 研究方法： NMR光谱 将样品溶于CD 3 OD中，在氮气流下干燥，然后溶于0.5 mL 2：1 CDCl 3-CD 3 OD中，置于5 mm NMR管中。在Varian Inova-500光谱仪上在25 ℃下获得一维和二维NMR光谱。 光谱宽度为2200 Hz。 使用标准Varian脉冲参数获得1-D质子、2-D gCOSY和2-D TOCSY实验。相对于残留的CHCl 3信号测量化学位移（相对于TMS =7.489 ppm）。进一步的实验细节可根据要求提供。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Methods: NMR Spectroscopy The sample was dissolved in CD3OD and dried under a stream of nitrogen and then dissolved in 0.5 mL 2:1 CDCl3-CD3OD and placed in a 5-mm NMR tube. One- and 2-dimensional NMR spectra were acquired on a Varian Inova-500 spectrometer at 25 ¿C. Spectral width was 2200 Hz. 1-D proton, 2-D gCOSY, and 2-D TOCSY experiments were acquired using standard Varian pulse parameters. Chemical shifts were measured relative to the residual CHCl3 signal (¿=7.489 ppm relative to TMS). Further experimental details are available upon request.