GRID AND SPECIMEN PREPARATION TECHNOLOGIES

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 本计画著重于发展新颖的栅极基板与低温电镜样品 制备技术该项目的具体目标是：（1）。 探索和评估新的 多孔碳箔的设计和几何形状，以优化数字化 - 图像采集和所采集图像的质量，目的是提高稳定性， 电导率和试样平整度。2.）的情况。系统地研究影响 大面积薄冰的产生，以及颗粒的方向和分布方式， 多孔网格3.）第三章探索和评估设计用于解决某些问题的新型样本支架 与碳支撑膜相关的问题。 目的是提供一种新的基底 具有改进的机械稳定性、导电性和平整度。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This projects focuses on the development of novel grid substrate and cryo-EM specimen preparation technologies. The specific aims of the project are: 1.) Explore and evaluate new designs and geometries for holey carbon foils in order to optimize the efficiency of digital image acquisition and the quality of the images acquired with the goal of improving stability, conductivity and specimen flatness. 2.) Systematically investigate factors that influence the production of large areas of thin ice, as well as the way particles orient and are distributed on the holey grids. 3.) Explore and evaluate novel specimen supports designed to address some of the problems associated with carbon support films. The aim is to provide a new substrate with improved mechanical stability, electrical conductivity and flatness.