MODEL OF PI3K/RHO-FAMILY GTPASE INTERPLAY DURING FIBROBLAST SPREADING

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 在我们实验室现有和计划的成像实验的成像数据的驱动下，通过细胞迁移联盟的合作，我们正在全细胞水平上建立PI 3 K/RFG共调节的空间模型。 一个唯象模型的信号突出耦合将被构建，活细胞成像实验的分析指导。 我们将尽可能在虚拟单元环境中开发模型或模块。 Haugh小组以前在我们的梯度传感工作中利用了VCell的新膜扩散能力，这些模型可供其他VCell用户公开使用。 细胞机械建模能力，目前正在开发的VCell团队与CMC建模倡议，将在这个项目中使用，因为他们成为可用的。这些将取决于下一个NRCAM项目期间计划的椭圆解算器。新提出的多物理场层和插件架构将促进将机制纳入VCell框架。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Driven by imaging data from existing and planned imaging experiments in our laboratory and through collaborations within the Cell Migration Consortium, we are building a spatial model of PI3K/RFG co-regulation at the whole-cell level. A phenomenological model of signaling-protrusion coupling will be constructed, guided by analysis of live-cell imaging experiments. To the extent possible, we will develop the model, or modules thereof, in the Virtual Cell environment. The Haugh group has previously made use of the new membrane diffusion capabilities of VCell in our work on gradient sensing, and these models are publicly available to other VCell users. Cell mechanical modeling capabilities, currently under development by the VCell team in collaboration with the CMC modeling initiative, will be utilized in this project as they become available. These will depend on the elliptic solver planned for the next NRCAM project period. Incorporation of mechanics into the VCell framework will then be facilitated by the newly proposed multiphysics layer and plug-in architecture.