INTRACELLULAR RNA TRAFFICKING

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 RNA运输是一个复杂的过程，其中特定的RNA定位于特定的亚细胞区室。通过各种体内和体外技术，我们已经描绘了一个分子相互作用的地图，描述在神经细胞中的RNA运输系统。我们已经测量了定量参数的每个分子间的相互作用，在RNA贩运使用表面等离子体共振分析在体外和荧光相关光谱（FCS）和活细胞。在RNA运输的虚拟细胞模型中使用定量参数。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. RNA trafficking is a complex process whereby specific RNAs are localized to particular subcellular compartments. Through a variety of in vivo and in vitro techniques we have delineated a molecular interaction map describing the RNA trafficking system in neural cells. We have measured quantitative parameters for each molecular interaction in RNA trafficking using surface plasmon resonance analysis in vitro and fluorescence correlation spectroscopy (FCS) and in live cells. Quantitative parameters are used in Virtual Cell models of RNA trafficking.