DEVELOPING X-RAY TOMOGRAPHY

这个子项目是许多利用资源的研究子项目之一 由NIH/NCRR资助的中心拨款提供。子项目的主要支持 而子项目的主要调查员可能是由其他来源提供的， 包括其它NIH来源。 列出的子项目总成本可能 代表子项目使用的中心基础设施的估计数量， 而不是由NCRR赠款提供给子项目或子项目工作人员的直接资金。 该核心致力于建立适用于以下方面的细胞和组织制备方法： 软X射线成像技术，简化样品处理和 X射线显微镜，并创建增强的以用户为中心的计算环境， 层析成像数据的有效处理和分析。主要实验 这个核心的一部分是开发方法来分类亚细胞的身份， 基于获得的形态和软X射线线性吸收曲线的组分 全细胞的三维断层重建。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. This core is dedicated to establishing cell and tissue preparation methods applicable to soft x-ray imaging, technology to streamline specimen handling and throughput on the x-ray microscope, and creating an enhanced user centric computational environment for the efficient processing and analysis of tomographic data. The major experimental component of this core is developing methods to classify the identities of sub-cellular components based on morphology and soft x-ray linear absorption profiles obtained from 3-D tomographic reconstructions of whole cells.