INTERACTIONS BETWEEN PROTEINS OF THE MEIOTIC SPINDLE POLE BODY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 在减数分裂过程中，纺锤体极体(SPB)的外部斑块发生重组，结构由微管生长部位转变为膜形成和锚定部位。这些膜被称为前孢子膜，对于孢子的形成是必不可少的。电子显微镜分析显示减数分裂外斑具有高度组织化的结构，双杂交分析发现这些外斑蛋白之间存在许多相互作用。然而，这些蛋白质在外部斑块中是如何相互组织的还不清楚。这些蛋白质在外部斑块中的排列将通过基于FRET的方法进行探索。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. During meiosis, the outer plaque of the spindle pole body (SPB) is reorganized.The structure is converted from a site of microtubule growth to one of membrane formation and anchorage. These membranes, termed prospore membranes, are essential for the formation of spores. Electron microscopy analysis reveals a very highly organized structure for the meiotic outer plaque, and two-hybrid analyses have identified many interactions between these outer plaque proteins. However, precisely how these proteins are organized with respect to each other within the outer plaque is not known. The arrangement of these proteins within the outer plaque will be explored by FRET-based methodology.