New methods for structural biology and drug discovery by nuclear magnetic resonance spectroscopy

顺磁性镧系元素标签为结构生物学和合理药物设计提供了新的机会。新的核磁共振（NMR）光谱技术将选择性地检测顺磁性镧系元素标记的蛋白质区域的NMR信号，加速蛋白质-配体复合物的结构分析。新的镧系元素标签将结合到磷酸丝氨酸和硒代半胱氨酸残基位点特异性引入蛋白质。这些标签也将使准确的距离测量的电子顺磁共振（EPR）光谱在大型，生物学上重要的蛋白质系统迄今不服从详细的结构研究和蛋白质经历构象变化。

Paramagnetic lanthanide tags offer fresh opportunities in structural biology and for rational drug design. Novel nuclear magnetic resonance (NMR) spectroscopy techniques will selectively detect the NMR signals from protein regions marked by paramagnetic lanthanides, accelerating the structure analysis of protein-ligand complexes. New lanthanide tags will bind to phosphoserine and selenocysteine residues site-specifically introduced into proteins. These tags will also enable accurate distance measurements by electron paramagnetic resonance (EPR) spectroscopy in large, biologically important protein systems hitherto not amenable to detailed structural studies and in proteins undergoing conformational changes.