Research on inhalation toxicology of environmental chemicals

在2011财年，为支持NTP研究的4名主要研究者进行了大约28项研究，为支持DIR研究（LRB）的5名主要研究者进行了大约89项研究。这些研究是为了支持大约13名博士后、研究生和其他非pi研究人员的研究而进行的。在2011财年，1873只动物（约20个小鼠品系和3个大鼠品系）被接收并维持在吸入设施用于这些研究。这些动物大多是用合同资金购买的。从杜克大学、NICHD、北卡罗来纳大学等来源接收了一些转基因小鼠品系，这些来源在主要校园动物设施中不被接受。另外，对未经批准来源的4株转基因小鼠建立了动物繁殖菌落。实验用大约13种不同的化学试剂（卵白蛋白、臭氧、高氧、2,3-丁二酮、乙酮、2,3-戊二酮、苯、磷化铟、氧化铟、巴霉素A1、高碳酸气体、甲胆碱、香烟烟雾）和大约7种生物化合物（脂多糖、癌症干细胞、树突状细胞、白喉毒素、透明质酸结合蛋白、脂质体、室内灰尘提取物）处理动物。化学制剂和生物制剂采用全身吸入、单鼻吸入、气管内滴注、口咽滴注、腹腔内注射和胸腔内注射。除了动物维护和治疗外，技术人员还进行了尸检，收集支气管肺泡液和组织进行组织病理学或分子分析，进行蛋白质和LDH分析，并使用Flexivent系统评估肺功能。

During FY2011 approximately 28 studies were conducted for 4 principal investigators in support of NTP research, and approximately 89 studies were conducted for 5 principal investigators in support of DIR research (LRB). These studies were conducted in support of research for approximately 13 post-docs, graduate students and other non-PI investigators. During FY2011, 1873 animals (~20 mouse strains and 3 rat strains) were received and maintained at the Inhalation facility for these studies. Most of these animals were purchased using contract funds. A number of genetically-modified mouse strains were received from sources (Duke, NICHD, UNC) that are not accepted at the main campus animal facility. In addition animal breeding colonies were set up for 4 strains of genetically-modified mice from unapproved sources. Animals were treated with approximately 13 different chemical agents (ovalbumin, , ozone, hyperoxia, 2,3-butanedione, acetoin, 2,3-pentanedione, benzene, indium phosphide, indium-tin-oxide, bafilomycin A1, hypercapnic gases, methacholine, cigarette smoke), and approximately 7 biological compounds (lipopolysaccharide, cancer stem cells, dendritic cells, diphtheria toxin, hyaluronan binding protein, liposomes, house dust extract). Chemicals and biological agents were administered by whole-body inhalation, nose-only inhalation, intratracheal instillation, oropharyngeal aspiration, intraperitoneal injection, and intrapleural injection. In addition to animal maintenance and treatment, technicians also performed necropsies, collected bronchoalveolar fluid, and tissues for histopathology or molecular analysis, conducted protein and LDH analyses and evaluated pulmonary function using the Flexivent system.