Monitor for Multiple Biomarkers for Detection of Alcohol Consumption

描述：我们建议开发一种新类型的监测仪，以直接和同时识别疑似接触酒精的人的多个生物标志物代谢物。我们计划使用的技术是表面增强拉曼散射(SERS)，基于一种著名的非弹性激光光散射分析技术，用于在实验室精确识别有机化合物。生物标记物检测器和基于生物标记物的检测必须灵敏、可重复性、价格实惠、可运输、可靠，并且检测器中使用的样本必须易于从血清、血浆、尿液、唾液或其他来源收集和获得。在这两种情况下，理想的检测设备都是一种小型、便携、易于使用、能够在临床环境中快速分析多个生物标志物的设备，具有最低限度的假阳性和假阴性，以避免浪费资源和错过实际暴露问题。通过迅速检测酒精生物标志物的存在和新出现的浓度，可以在暴露周期的早期识别酒精对组织和内脏的威胁，从而扩大高危人群的治疗选择。拟议的研究计划旨在利用SERS光谱在单个现场可部署设备中对多个生物标记物进行快速现场血液、尿液或唾液分析，将拉曼技术的强大功能以实用、经济高效的配置带到现场临床分析中。

DESCRIPTION: We propose to develop a new type of monitor to directly and simultaneously identify multiple biomarker metabolites in persons suspected of having been exposed to alcohol. The technology we plan to utilize is surface-enhanced Raman scattering (SERS), based on a well-known inelastic laser light scattering analytical technique for precise lab identification of organic compounds. Biomarker detectors and biomarker-based tests must be sensitive, reproducible, affordable, transportable, reliable, and the samples employed in the detectors must be easy to collect and obtain from serum, plasma, urine, saliva, or other sources. The ideal detection device in both cases is one that is small, portable, easy to use and capable of rapid analysis of multiple biomarkers in a clinical setting, with a minimum of false positives and false negatives to avoid wasting resources and missing actual exposure problems. By detecting the presence of the alcohol biomarkers promptly and at emerging concentrations, the alcohol threat to tissue and internal organs may be identified early in the exposure cycle, expanding treatment options in the population at risk. The proposed research program seeks to utilize SERS spectroscopy for rapid at-site blood, urine or saliva analysis for multiple biomarkers in a single, field-deployable device, bringing the power of the Raman technique in a practical, cost-effective configuration to at-site clinical analysis.