3rd U.S.-Canada Winter School on Biomolecular Solid State NMR

描述(申请人提供)：在过去的10-15年里，固态核磁共振(SS核磁共振)作为一种技术出现了，它产生了关于不能用X射线衍射或溶液核磁共振检查的系统的重要结构数据，这两个目前是结构生物学的两大支柱。例如，SS核磁共振实验最近产生了一些膜多肽和蛋白质的第一个结构，以及第一个由实验确定的淀粉样纤维的结构，这些结构一直不能用其他技术进行详细研究，因为它们不能溶解，也不会在其生物相关的状态下结晶。最近的这些成功是光谱和样品制备方法的根本和实际改进的结果。与此同时，固体核磁共振发展中出现的技术，例如平均哈密顿理论，也影响了其他研究领域，如溶液核磁共振和磁共振成像。最后，通过动态核极化(DNP)提高灵敏度的复兴，最初是为了SS核磁共振实验，现在正在溶液核磁共振和成像中得到广泛应用，并进一步将高场电子顺磁共振(EPR)和核磁共振集成到一个单一的流形中。为了向科学界迅速传播这些进展，我们建议于2013年1月6日至11日在佛蒙特州斯托举办第三届生物分子固体核磁共振冬季学校。该学院的主要组成部分将是由该领域的领先研究人员就基础和高级主题进行的一系列讲座。主题将包括现代SS核磁共振数学理论的基本方面，特定最先进实验技术的实用优势的评估，同位素标记蛋白质和多肽的制备方法，数据处理和数值模拟，根据SS核磁共振数据开发结构模型等。课程将通过问题集和软件(SPINVOLUTION，Simpson，GAMMA等)的动手演示来补充。目前用于模拟对生物固体很重要的脉冲核磁共振实验。参加这所学校将使研究生和博士后学生接触到广泛的基本技术和概念，极大地加强他们原本通过自己研究小组的具体研究项目获得的更专门的培训。这是第三所SS核磁共振学校，是为了回应我们从2008年1月和2010年1月就读于第一和第二学校的学生和博士后那里收到的非常积极的反馈而组织的。因此，我们认为冬季学校对于蓬勃发展的固态生物核磁共振社区来说是一个极其重要的教学活动，因此与日历上过多的其他会议不同。

DESCRIPTION (provided by applicant): In the past 10-15 years, solid state NMR (SSNMR) has emerged as a technique that yields important structural data on systems not amenable to examination with either X-ray diffraction or solution NMR, presently the two pillars of structural biology. For example, SSNMR experiments have recently produced some of the first structures of membrane peptides and proteins and the first experimentally-determined structures of amyloid fibrils, which have been resistant to detailed investigation by other techniques because they are insoluble and do not crystallize in their biologically relevant state. These recent successes are a result of fundamental and practical improvements in spectroscopic and sample preparation methods. Concurrently, technology emerging from developments in SSNMR, for example Average Hamiltonian Theory, has influenced other areas of research such as solution NMR and magnetic resonance imaging. Finally, the renaissance in sensitivity enhancement via dynamic nuclear polarization (DNP) originally intended for SSNMR experiments is finding wide application in solution NMR and imaging and is further integrating high field electron paramagnetic resonance (EPR) and NMR into a single manifold. In order to rapidly propagate these advances to the scientific community we propose to hold the 3rd Winter School on Biomolecular Solid State NMR in Stowe, VT January 6-11, 2013. The principal component of this school will be a series of lectures on fundamental and advanced topics by leading researchers in the field. Topics will include basic aspects of the mathematical theory of modern SSNMR, assessment of practical advantages of specific state-of-the-art experimental techniques, methods for preparation of isotopically labeled proteins and peptides, data processing and numerical simulation, the development of structural models from SSNMR data, etc. The lectures will be supplemented by problem sets and hands-on demonstrations of software (SPINEVOLUTION, SIMPSON, GAMMA, etc.) currently used to simulate pulsed NMR experiments important for biological solids. Attendance at this school will expose graduate and postdoctoral students to a broad spectrum of essential techniques and concepts, greatly enhancing the more specialized training they would otherwise obtain through specific research projects in their own research groups. This is the 3rd SSNMR school and is being organized in response to the very positive feedback that we received from the students and postdoctorals that attended the 1st and 2nd schools in January 2008 and 2010. As a result we believe the Winter School an extremely important pedagogical event for the blossoming solid-state biological NMR community, and as such differs from the plethora of other meetings on the calendar.