Analytical Cytometry

项目概述（见说明）：分析细胞术中心（ACC）的目标是通过其设施、尖端设备和经验丰富的操作人员，为COHCCC成员提供细胞及其成分的特性测量，分离这些细胞及其成分，并提供获得的数据用于内部分析和外部审查。流式细胞仪包括：3台高速细胞分选仪（MoFlo， BD FACS Aria SORP和BD FACS Aria 111）和4台分析细胞仪（Cyan, Gallios， LSR Fortessa和C6）。流式细胞仪仪器为研究人员提供了分析和分离细胞的工具，以高达20,000个细胞/秒的速度，基于多个荧光标记（高达18个）和光散射特性，具有高产率（基于速度高达90%）和极高纯度（99%）。此外，BD Aha 11 SORP包含在生物安全柜中，允许用户对活（潜在传染性）样品进行分类。ACC的所有仪器都要接受每日（sorters和Fortessa）或每周（Gallios， CyAn和C6）的质量控制评估和例行预防性维护和校准。行政协调会产生的所有数据均可通过机构的网络基础设施网获得，以供进一步分析和准备提出或出版。核心提供了基于网络的数据处理软件，并开发了实验室信息管理系统（LIMS），以帮助跟踪实验相关的元数据和存档文件检索。ACC的另一个关键作用是为COHCCC成员提供流式细胞仪的基础理论、实验设计、软件使用和操作方面的培训。

PROJECT SUMMARY (See instructions): The goal of the Analytical Cytometry Core (ACC) is to provide the COHCCC members, through its facilities, leading-edge equipment and experienced operators to measure properties of cells and their components, isolate those cells and their components and present the data acquired for internal analysis and external review. Flow cytometry instrumentation available through this core resource includes: 3 high speed cell sorters (MoFlo, BD FACS Aria SORP and BD FACS Aria 111), and 4 analytical cytometers (Cyan, Gallios, LSR Fortessa and C6). The flow cytometry instrumentation provides investigators with the tools to analyze and isolate cells at speeds of up to 20,000 cells/second based on multiple fluorescent labels (up to 18) and light scatter properties with high yield (up to 90% based on speed) and extreme purity (99%). Additionally, the BD Aha 11 SORP is contained in a biosafety cabinet and allows users to sort live (potentially infectious) samples. All instrumentation in the ACC is subject to either daily (sorters and Fortessa) or weekly (Gallios, CyAn, and C6) quality control assessment and routine preventive maintenance and calibration. All data generated in the ACC is available through the institutional cyber-infrastructure network for further analysis and preparation for presentation or publication. Network based data processing software is offered by the core and a Laboratory Information Management System (LIMS) is being developed to help track experiment related meta data and archived file retrieval. Another key role of ACC is to provide flow cytometry training from basic theory, experimental design, software usage and operation of the cytometers to COHCCC members.