Identification and Quantification of Sequence Specific RNA Modifications via the Alpha-Hemolysin Latch Zone

描述(由申请人提供)：在这项STTR计划中，电子生物科学(EBS)建议围绕我们犹他大学的合作者开发一个基于纳米孔的系统，新开发的技术将能够识别和量化序列特定的RNA修改。一个能够定量、低成本、高通量地表征RNA修饰的系统有能力阐明这些修饰在细胞信号中的作用，并提高我们对基因调控和相关疾病状态的理解。此第一阶段计划的目标是演示RNA修饰表征概念的概念验证，并确定使我们的团队能够在后续第二阶段计划期间开发完整的RNA修饰表征系统的参数。

DESCRIPTION (provided by applicant): During this STTR program, Electronic BioSciences (EBS) proposes to develop a nanopore based system around our University of Utah collaborators newly developed technology that will be capable of identifying and quantifying sequence specific RNA modifications. A system capable of quantitative, low cost, high throughput, RNA modification characterization has the capability to elucidate the roles of these modifications in cellular signaling, and improve our understanding of gene regulation and associated disease states. The goal of this Phase I program is to demonstrate proof-of-concept for the RNA modification characterization concept, and determine the parameters that will allow our team to develop a complete RNA modification characterization system during a follow-on Phase II program.