Core D: Research Support (Anderson)

项目总结(见说明)： 研究界和监管界认识到有必要开发多环芳烃的分析方法并取代多环芳烃(x-PAHs)。最近美国环保局IRIS 2010年的文件《多环芳烃混合物的相对效力工厂方法的开发》(Development of a Relative Pigency Factory，RFP)指出，虽然首选的是全混合物评估……但混合物成分的化学分析是有限的……RPF方法受到分析化学所涉及的少量多环芳烃的限制。环境和生物基质中的多环芳烃和x-多环芳烃的分析是具有挑战性的，因为样品复杂，烷基化和高分子质量化合物的众多异构体往往难以区分，而且我们往往缺乏标准和合适的参考物质。我们将从适用的基质中提供混合物的快速化学筛选和定量分析。化学核心为超级基金中心的调查人员提供关键的专业分析专业知识。我们将开发多环芳烃和混合物的先进技术和方法。对新的分析方法的需求继续排在机构工作组列举的数据差距清单的首位。我们需要进一步发展我们的1,201种分析物筛选方法，以“了解你的环境”，与基因组“了解你自己的技术进步”。我们将继续开发一个合成装置，以制作商业上无法获得的化学标准。我们通过合成多环芳烃代谢物使我们的生物医学项目得以开展。我们计划扩大我们的合成成分，以包括多环芳烃和取代的多环芳烃，这些化合物目前还没有商业用途。缺乏商业标准构成了分析方法开发和随后的生物医学测试的关键障碍。我们将继续开发基于暴露的混合参考物质、标准和被动采样设备的集中存储库。我们建议开发新的超级基金参考材料，包括基于生物可用超级基金的材料以及补救前后的提取物。我们将向SRP调查人员提供这些具有良好特性的混合物。我们还将准备和分发被动采样设备给SRP调查人员和美国环保局项目经理，以进行试点调查和评估。

PROJECT SUMMARY (See instructions): The research and regulatory communities acknowledge the need to develop analytical methods for PAHs and substitute PAHs (x-PAHs). The recent US EPA IRIS 2010 document Development of a Relative Potency Factory (RFP) Approach for Polycyclic Aromatic Hydrocarbon Mixtures says, "While whole mixture assessment is preferred . . . chemical analysis of the components of mixtures is limited . . . The RPF approach is limited by the small number of PAHs for which there are analytical chemistry." It is challenging to analyze PAHs and x-PAHs in environmental and biological matrices because the samples are complex, the numerous isomers of alkylated and high-molecular-mass compounds are often difficult to differentiate, and we often lack standards and suitable reference materials. We will provide rapid chemical screening and quantitative analysis of mixtures from applicable matrices. The Chemistry Core provides Superfund Center Investigators with critical specialized analytical expertise. We will develop advanced techniques and methods for PAHs and mixtures. The need for new analytical methods continues to top the list of data gaps cited by agency workgroups. We need to further develop our 1,201 analyte screening method to "know thy environment" paralling the genome "know thyself technical advances. We will continue to develop a synthesis unit to make chemical standards that are not commercially available. We enable the work of our biomedical projects by synthesizing PAH metabolites. We plan to expand our synthesis component to include PAHs and substituted PAHs that are not currently commercially available. The lack of commercial standards constitutes a critical barrier to analytical method development and subsequent biomedical testing. We will continue to develop a centralized repository for exposure-based mixture reference materials, standards, and passive sampling devices. We propose to develop new Superfund reference materials, including bioavailable Superfund based materials and pre-and post-remediation extracts. We will make these well-characterized mixtures available to SRP investigators. We will also prepare and distribute passive sampling devices to SRP investigators and US EPA project managers for pilot investigations and evaluation.