Molecular Core

我们的Udall中心提案继续拥有一个分子核心，旨在为重新 搜索队。分子核心将为项目团队提供四项服务。第一个是提供基因 荧光激活细胞分选与实时定量相结合的基因表达分析 聚合酶链式反应(QPCR)。项目1、2、3和5使用这种方法。第二项服务是提供咨询 并协助进行基因分型。项目1、2、3和4广泛使用转基因动物，这些动物需要 准确、及时的基因分型。第三项服务是在病毒的设计和建造方面提供帮助 基因敲除载体。项目2和项目3都广泛使用了这项技术。第三项服务是 为基因传递的病毒载体的设计和构建提供帮助。项目1、2、3和5使 使用该服务递送与光遗传学、药物基因组学和 氧化还原报道。陈冯富珍博士将担任核心董事，并监督核心的运作。陈博士 在基因表达研究方面有丰富的经验，包括：单细胞，基于FACS的细胞群体，以及 组织水平分析。分子核设施中的所有必要方法都已在先前建立。这个 分子核心将由陈博士在Jyothisri Kondapalli博士的协助下进行管理。在一起，他们 将负责核心的日常运营，监督技术人员，采购用品等。

Our Udall Center proposal continues to have a Molecular Core intended to provide scientific support to the re- search teams. The Molecular Core will provide four services to the program team. The first is to provide gene expression analysis using a combination of fluorescence activated cell sorting (FACS) and quantitative real time PCR (qPCR). Projects 1, 2, 3, and 5 make use of this approach. The second service is to provide consultation and assistance for genotyping. The projects 1, 2, 3 and 4 makes extensive use of transgenic animals that require accurate and timely genotyping. The third service is to provide assistance in the design and construction of viral vectors for gene knockdown. Both projects 2 and 3 make extensive use of this technology. The third service is to provide assistance in the design and construction of viral vectors for gene delivery. Projects 1, 2, 3 and 5 make use of this service for delivery of various expression constructs related to optogenetics, pharmacogenomics and redox reporting. Dr. C. Savio Chan, will serve as the Core Director and oversee the operation of the core. Dr. Chan has extensive experience with gene expression studies, including: single-cell, FACS-based cell-population, and tissue-level analyses. All necessary methods in the Molecular Core facility have been established previously. The Molecular Core will be administered by Dr. Chan with the assistance of Dr. Jyothisri Kondapalli. Together, they will be responsible for the day-to-day operation of the core, supervision of technical staff, purchasing supplies, etc.