Biorepository of cancer tissue samples from WTC responders

描述（由申请人提供）：有人担心世界贸易中心（WTC）反应者的癌症发病率可能会增加。然而，众所周知，大多数职业性癌症在接触致癌物质后一个或多个十年才变得明显;此外，该队列在组装时相对年轻。9/11事件十周年是研究这一人群癌症发生率的一个重要里程碑，因为暴露后的时间范围（10年）与癌症的病因相关，而且这一群体正在老龄化，因此进入了一个癌症发生的时期。 变得更加频繁。现在需要用癌症组织库来补充当前的流行病学癌症随访;生物标本资源及其临床注释是推动转化研究的一些最强大的资源之一。我们建议为WTC新诊断的癌症实施一个组织库系统，重点是促进对这些肿瘤生物学的理解。该项目将启动和管理一个癌症组织样本和邻近正常组织的中央储存库，这些样本来自WTCHP参与者中诊断出的每种实体癌。每种癌症的病理学和细胞学报告的相关信息也将集中存储。此存储库将能够 与WTCHP主要数据集相关联，该数据集包含在纳入WTCHP时和定期进行的随访期间为参与者收集的临床、流行病学和暴露信息。随后，将从每个组织样本中制备RNA、DNA和组织微阵列（TMA）。这些样本将可用于未来的基因组研究、基因-环境相互作用研究以及DNA甲基化研究。也可以对储存的样本进行特定分析，如免疫组织化学、原位杂交、DNA倍性分析、核形态测定和荧光原位杂交。我们还将建立组织库，作为科学界的资源，为合格的申请人定义一个程序，以请求可用的样本用于研究，并访问数据库中的流行病学和临床信息。组织库将代表解决特定致癌物暴露与某些癌症部位之间的联系，可能与癌症有关的暴露分子特征，WTC反应者中肿瘤侵袭性的特定标志物等问题的必要基础设施。这将最终影响治疗方式，以及这些患者的成功和生存概率。

DESCRIPTION (provided by applicant): There has been concern about the possibility of an increase in cancer incidence among the World Trade Center (WTC) responders. However, it is known that most occupational cancers become manifest only one or more decades after carcinogenic exposure; additionally, the cohort was relatively young at the time it was assembled. The tenth anniversary of 9/11 marks a significant landmark for studies on cancer occurrence in this population, since the time frame after the exposure (10 years) is becoming etiologically relevant for cancer, and the cohort is aging thus entering a time in life when cancer becomes more frequent. There is now a need to supplement the current epidemiologic cancer follow-up with a cancer tissue bank; biospecimen resources and their clinical annotations are among some of the most powerful resources fueling translational research. We propose to implement a tissue bank system for the WTC newly diagnosed cancers, focused on advancing the understanding of the biology of these tumors. This project will initiate and manage a central repository of cancer tissue samples and adjacent normal tissue from each solid cancer diagnosed among the WTCHP participants. Relevant information from pathology and cytology reports from each cancer will also be centrally stored. This repository will have the capability to be linked with the main WTCHP data set containing clinical, epidemiological and exposure information collected for the participants at the time of inclusion in the WTCHP and during the regularly performed follow up. Subsequently, RNA, DNA and tissue microarrays (TMA) will be prepared from each tissue sample. Such samples will be made available for future genomic research studies, studies of gene-environment interaction, as well as DNA methylation studies. Specific analyses such as immunohistochemistry, in situ hybridization, DNA ploidy analysis, nuclear morphometry, and fluorescent in situ hybridization will also be possible on the stored samples. We will also establish the tissue bank as a resource for the science community, by defining a process for qualified applicants to request available samples for research use, and for access epidemiologic and clinical information in the database. The tissue bank will represent the necessary infrastructure for addressing questions such as the link between specific carcinogens exposures and certain cancer sites, molecular signatures of exposure that could be linked to cancer, specific markers of tumor aggressiveness among WTC responders.. This will ultimately impact the modalities of treatment, and the probability of success and survival of these patients.