Real-time assessment of surgical margins with enhanced contract optical imaging

描述(由申请人提供)：物理科学公司(PSI)与马萨诸塞州总医院合作，提议研究使用一种高灵敏度/特异性的癌症靶向剂和一种新的荧光引导显微镜(FGM)方案来评估乳腺癌患者的手术切缘。造影剂将被用来标记癌细胞，并在肿块切除标本上突出阳性边缘。然后，女性生殖器切割将被用来在细胞尺度上可视化组织形态，并为病理学家提供类似组织学的图像，以确认或排除癌症的存在。在第一阶段，我们建议开发、测试和优化适用于癌症标记的造影剂和台式仪器，该仪器将在同一光学平台中结合荧光成像和高分辨率/穿透深度相干显微镜，并具有自动双模式图像配准。这项技术的初步测试将在几个外科标本上进行。在第一阶段结论的基础上，这项技术将在第二阶段研究中得到改进，并在马萨诸塞州总医院的临床环境中进行测试。

DESCRIPTION (provided by applicant): Physical Sciences, Inc. (PSI), in collaboration with Massachusetts General Hospital proposes to investigate the use of a high sensitivity/specificity cancer targeting agent and of a novel fluorescence-guided microscopy (FGM) scheme for intraoperative assessment of surgical margins in breast cancer patients. The contrast agent will be used to label cancer cells and highlight positive margins on lumpectomy specimens. FGM will then be used to visualize tissue morphology at the cellular scale and provide the pathologist with histology-like images to confirm or rule out cancer presence. In Phase I we propose to develop, test, and optimize a suitable contrast agent for cancer labeling and a benchtop instrument that will combine fluorescence imaging and high resolution/penetration depth coherence microscopy within the same optical platform with automated dual modal image registration. Preliminary testing of this technology will be performed on several surgical specimens. Based on Phase I conclusions, this technology will be improved during a Phase II study and tested in a clinical setting at the Massachusetts General Hospital.