Clinical Research and Assay Support

临床研究核心负责处理大量正在进行的临床研究以及已经完成的研究中的人体样本。一旦采集到样本，我们就会根据规程上的说明进行处理，并根据首席调查员的指示对样本进行等量、标记和储存。我们目前平均每周处理1200件样品。在实验室处理的所有样品都设置了跟踪号，并输入了由Core实验室维护的基于Web的数据库。自2008年1月开始的研究使用2D条形码系统进行标记和跟踪。Core实验室目前储存了250K的人类生物标本。Core还为美国国内以及国际上的PI运送和跟踪人类生物标本。 除了生物信息库服务外，临床研究核心还为DLm目前无法提供的分析提供分析支持。我们已经测试和验证了多种方法来检测人类血浆中总的和活性的Glug样肽-1、酰基Ghrelin、Desacyl Ghrelin、Leptin、脂联素、Oxyntomodrin和其他几种标志物。 临床核心还分离了几项研究的基因组DNA，这些研究需要gDNA进行基因测试。 该实验室还利用Parr Bomb热量计提供测试服务。弹式量热服务提供了一种获取液体和固体样品燃烧热的方法。我们目前正在开发一种用于粪便、尿液和食物样本的热量分析方法。

The Clinical Research Core is responsible for processing human samples from numerous on going clinical studies as well as studies that have been completed. Once the samples are acquired we process it according to the instructions on the protocol, aliquot, label and store the samples for the various principal investigators as per their instructions. We are currently processing an average of 1200 samples/week. All samples processed in the lab are set up with a tracking number and entered into a web based database which is maintained by the Core lab. Studies that have started since January 2008 are labeled and tracked using a 2D-barcode system. The Core lab currently stores 250K human biospecimen. The Core also ships and tracks human biospecimen for the PIs within the US as well as internationally. In addition to the biorepository services, the Clinical Research Core also provides assay support for analyses which are currently not available at DLM. We have tested and validated numerous methods to measure total and active Glucagon-like Peptide-1, Acyl Ghrelin, Desacyl Ghrelin, Leptin, Adiponectin, Oxyntomodulin and several other markers in human plasma. The Clinical core also isolates genomic DNA for several studies where gDNA is required for gene testing. The lab also provides a testing service utilizing a Parr Bomb Calorimeter. The bomb calorimetry service provides a method for obtaining heats of combustion for liquid and solid samples. We are currently developing a method for calorimetric analysis of fecal, urine and food samples.