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Clinical Research and Assay Support

临床研究和检测支持

基本信息

批准号：
10904550
负责人：
Mary Walter
金额：
$ 125.86万
依托单位：
NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10904550
关键词：
Accounting Aliquot Bar Codes Biological Biological Assay Biological Markers Biological Specimen database Blood Clinical Clinical Research Collaborations Collection Data Databases Diabetes Mellitus Endocrine Ensure Enteroglucagon Genomic DNA Hormones Hour Human Instruction International Label Leptin Liquid substance Measurement Measures Metabolic Methods National Institute of Diabetes and Digestive and Kidney Diseases Online Systems Oxidative Stress Plasma Preparation Principal Investigator Process Protocols documentation Reproducibility Research Research Personnel SARS-CoV-2 antibody Sampling Serology Services Ships Site Specimen Statutes and Laws System Technology Testing Tube Whole Blood adipokines adiponectin biobank cytokine des-n-octanoyl ghrelin genetic testing ghrelin glucagon-like peptide 1 preservation programs web platform

项目摘要

The Clinical Research Core is responsible for processing human samples from numerous on going clinical studies as well as studies that have been completed. Once the samples are acquired we process it according to the instructions on the protocol, aliquot, label and store the samples for the various principal investigators as per their instructions. We are currently processing an average of 1200 samples/week. All samples processed in the lab are set up with a tracking number and entered into a web based database which is maintained by the Core lab. Studies that have started since January 2008 are labeled and tracked using a 2D-barcode system. The Core lab currently stores 250K human biospecimen. The Core also ships and tracks human biospecimen for the PIs within the US as well as internationally. In addition to the biorepository services, the Clinical Research Core also provides assay support for analyses which are currently not available at DLM. We have tested and validated numerous methods to measure total and active Glucagon-like Peptide-1, Acyl Ghrelin, Desacyl Ghrelin, Leptin, Adiponectin, Oxyntomodulin and several other markers in human plasma. Recently we have added serology and neutralization assays against antibodies to SARS-CoV-2. The Clinical core also isolates genomic DNA for several studies where gDNA is required for gene testing.

临床研究核心负责处理来自大量正在进行的临床研究以及已完成的研究的人体样本。一旦获得样本，我们就会根据方案上的说明对其进行处理，按照各主要研究人员的指示等分、标记和存储样本。目前我们平均每周处理 1200 个样品。实验室处理的所有样品都设置有跟踪编号，并输入到由核心实验室维护的基于网络的数据库中。自 2008 年 1 月开始的研究使用二维条形码系统进行标记和跟踪。核心实验室目前储存了 25 万份人类生物样本。 Core 还在美国和国际范围内为 PI 运送和跟踪人类生物样本。除了生物样本库服务外，临床研究核心还为 DLM 目前无法提供的分析提供分析支持。我们已经测试和验证了多种方法来测量总的和活性的胰高血糖素样肽-1、酰基生长素释放肽、去酰基生长素释放肽、瘦素、脂联素、泌酸调节素和人血浆中的几种其他标记物。最近，我们增加了针对 SARS-CoV-2 抗体的血清学和中和测定。临床核心还分离基因组 DNA，用于基因测试需要 gDNA 的多项研究。

项目成果

期刊论文数量（12）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Ingestion of diet soda before a glucose load augments glucagon-like peptide-1 secretion.

DOI：
10.2337/dc09-1185
发表时间：
2009-12
期刊：
Diabetes care
影响因子：
16.2
作者：
Brown RJ;Walter M;Rother KI
通讯作者：
Rother KI

Erythropoietin reduces fat mass in female mice lacking estrogen receptor alpha.

DOI：
10.1016/j.molmet.2020.101142
发表时间：
2021-03
期刊：
Molecular metabolism
影响因子：
8.1
作者：
Lee J;Walter MF;Korach KS;Noguchi CT
通讯作者：
Noguchi CT

Pigment Epithelium-Derived Factor Declines in Response to an Oral Glucose Load and Is Correlated with Vitamin D and BMI but Not Diabetes Status in Children and Young Adults.

色素上皮衍生因子因口服葡萄糖负荷而下降，并且与维生素 D 和 BMI 相关，但与儿童和年轻人的糖尿病状况无关。

DOI：
10.1159/000466692
发表时间：
2017
期刊：
Hormone research in paediatrics
影响因子：
3.2
作者：
Sylvetsky,AllisonC;Issa,NajyT;Chandran,Avinash;Brown,RebeccaJ;Alamri,HussamJ;Aitcheson,Gabriella;Walter,Mary;Rother,KristinaI
通讯作者：
Rother,KristinaI

Effects of diet soda on gut hormones in youths with diabetes.

DOI：
10.2337/dc11-2424
发表时间：
2012-05
期刊：
Diabetes care
影响因子：
16.2
作者：
Brown RJ;Walter M;Rother KI
通讯作者：
Rother KI

Limited cross-variant immune response from SARS-CoV-2 Omicron BA.2 in naïve but not previously infected outpatients.

DOI：
10.1016/j.isci.2022.105369
发表时间：
2022-11-18
期刊：
ISCIENCE
影响因子：
5.8
作者：
Lee, Hye Kyung;Knabl, Ludwig;Walter, Mary;Furth, Priscilla A.;Hennighausen, Lothar
通讯作者：
Hennighausen, Lothar