Core B: Lipidomics Core(Consortium)

项目总结 PPG中的脂质组学共享资源核心(LSR Core)的目的是提供智力和 物理资源(特别是最新的鞘脂和合成的质谱分析) 研究脂质的分子工具)，以加强我们对生物活性脂质在癌症生物学中的作用的理解 目标是发现未来的药物。生物活性脂类的多样性及其相互联系的代谢 产生一个调节细胞内和细胞间信号和功能的通路网络。功能障碍 这些途径有助于癌症进展和转移的病理生物学。这些考虑因素 这就需要脂质化学和分析的发展。LSR核心是基于以下条件创建的 关键人员在脂类化学、分析和代谢方面的独特专业知识。它已经演变成一把钥匙 项目赠款(PPG)的核心，题为鞘脂在癌症生物学和治疗中，并被 由所有参与调查的人员使用。此外，这个核心已经发展成为一个制度性的、全国性的和 脂类组学和脂类化学生物学新兴领域的国际资源。核心服务包括：1) 提供来自不同生物材料(细胞、 组织和生物液体)，主要使用高效液相色谱-串联质谱仪 光谱分析(LC-MS/MS)技术-Core目前提供超过300个 基础代谢水平上不同的脂类分子种类；2)开发和提供合成分子 研究脂质代谢的工具(例如，功能化和荧光神经酰胺、部位特异性放射性 鞘磷脂、17C-鞘磷脂)和用于细胞、体外和体内的各种合成脂质和类似物 研究(例如，细胞器靶向的鞘脂和细胞器靶向的鞘脂代谢抑制剂 酶)；以及3)协助PPG研究人员进行实验设计，选择感兴趣的脂类，以及 分析结果的解释。LSR核心对PPG的成功起到了重要作用 通过为他们提供独特的能力和关键结果，在过去10年中 继续为此次PPG续签的成功进展做出贡献。

PROJECT SUMMARY The purpose of the Lipidomics Shared Resource Core (LSR Core) in the PPG is to provide intellectual and physical resources (particularly state-of-the-art mass spectrometry analysis of sphingolipids and synthetic molecular tools to study lipids) to enhance our understanding of the role of bioactive lipids in cancer biology with the goal to discover future drugs. The diversity of bioactive lipids and their interconnected metabolism generates a network of pathways regulating intra- and inter-cellular signaling and function. Dysfunctions in these pathways contribute to the pathobiology of cancer progression and metastasis. These considerations have necessitated the development of lipid chemistry and analysis. The LSR Core was created based on unique expertise of the key personnel in lipid chemistry, analysis, and metabolism. It has evolved into a key Core for the program project grant (PPG) entitled Sphingolipids in Cancer Biology and Therapeutics, and was used by all participating investigators. Furthermore, this Core has developed into an institutional, national, and international resource in the emerging fields of lipidomics and lipid chemical biology. Core services include: 1) providing qualitative and quantitative analysis of lipid components from different biological materials (cells, tissue, and biological fluids), primarily employing high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technology-the Core currently provides quantitative analysis of more than 300 distinct lipid molecular species at a basic metabolomic level; 2) developing and providing synthetic molecular tools to study lipid metabolism (e.g., functionalized and fluorescent ceramides, site-specific radioactive sphingolipids, 17C-sphingolipids) and diversified synthetic lipids and analogs for cellular, in vitro, and in vivo studies (e.g., organelle-targeted sphingolipids and organelle-targeted inhibitors of sphingolipid metabolizing enzymes); and 3) assisting PPG investigators in experimental design, selection of lipids of interest, and interpretation of analytical results. The LSR Core has been instrumental to the success of the PPG investigators over the past 10 years by providing them with unique capabilities and critical results that should continue to contribute for the successful progress of this PPG renewal.