Basic-Genomics High-Throughput Facility (GHTF)

核心005-基本-项目摘要/摘要-基因组学高通量设备(GHTF) 生物医学发生了一场革命，基因组学信息使人们能够更快、更准确地 为患者提供诊断和精确的治疗方法。这场革命是由以下方面的发展引发的 空间时代设备的小型化以实现高通量、化学和光谱技术的进步 标签，并使用计算机技术来推进基于芯片的产品和并行数据处理 支持个性化计算的通信。这场革命是由技术驱动的。的第一个任务 作为赵氏家族综合资源共享资源的基因组高通量设备(GHTF) 癌症研究中心(CFCCC)将充当引进新兴基因组学技术的渠道，以便 最广泛的研究人员可以有效地访问它们。对这一共享资源的投资 该中心利用了国家资助的文书赠款和校园费用分摊。这些 投资已使Affymetrix微阵列、Illumina HiSeq下一代测序和PacBio排名第三 癌症研究人员可在介绍后一到两年内进行世代测序 工作空间--早在它们以实验定制的形式商业化之前很久。这些 技术使最先进的研究在很短的时间内转化为 转录因子、选择性剪接和聚腺苷酸化在癌症中心的意外作用 研究人员。在校园层面引入这些技术具有额外的刺激价值 促进这些技术的进一步跨学科发展的协作和反馈。第二 GHTF的使命是培训新一代基因组数据分析方面的研究人员。在当前 在资助期间，建立了HiSeq和PacBio测序的DNA测序分析管道。一个 实施了生物信息学咨询服务，以确保这些数据对癌症研究的影响。钥匙 GHTF对CFCC研究人员未来的发展重点是：1)开发单细胞分析 2)拓展生物信息学培训与分析。肿瘤种群是异质性的 要有效地研究它们，就需要有区分它们的能力。GHTF将 介绍一种用于从单个细胞中制备测序文库的微流控系统。有能力 分析大量数据流可以说是目前将基因组学应用于癌症的限制技术 研究。在本供资期间，我们在这方面取得了实质性进展，现在将扩大这些 为初级和高级应用程序量身定做的更长时间和更专业的研讨会，以及其他 工作人员的时间用于生物信息学，以及访问与癌症相关的途径数据库。

Core 005 - Basic - Project Summary/Abstract - Genomics High-Throughput Facility (GHTF) A revolution has occurred in biomedicine in which genomics information is enabling faster and more accurate diagnoses and precise tailoring of therapeutics to patients. This revolution was triggered by developments in space age miniaturization of devices to enable high throughput, chemistries and spectroscopic advances for labeling, and use of computer technologies to advance chip-based products and parallel data processing and communications to enable personalized computing. This revolution is technology-driven. The first mission of the Genomics High Throughput Facility (GHTF) as a shared resource of the Chao Family Comprehensive Cancer Research Center (CFCCC) is to act as a conduit to introduce emerging genomics technologies so that they can be effectively accessed by the broadest spectrum of researchers. Investment in this shared resource by the CFCCC has been leveraged by nationally-funded instrument grants and campus cost-sharing. These investments have made Affymetrix microarrays, Illumina HiSeq next generation sequencing and PacBio third generation sequencing available to cancer researchers within one or two years of introduction to the workspace--long before they were commercially available for experimentally-tailored formats. These technologies enabled state-of-the-art research which has translated in a very short time into identification of unexpected roles for transcription factors, alternative splicing and polyadenylation by Cancer Center researchers. Introduction of the technologies at the campus level has the additional value of stimulating collaborations and feedback into further interdisciplinary development of these technologies. The second mission of the GHTF is to train a new generation of researchers in the analysis of genomics data. In the current funding period, DNA sequencing analysis pipelines for HiSeq and PacBio sequencing were established. A Bioinformatics Consulting Service was implemented to insure impact of these data on cancer research. Key future development priorities for CFCC researchers by the GHTF are: 1) development of a single-cell analysis platform and 2) expansion of bioinformatics training and analysis. Tumor populations are heterogeneous collections of cells and that to study them effectively requires the ability to discriminate among them. GHTF will introduce a microfluidic system for preparation of sequencing libraries from individual cells. The ability to analyze large streams of data is arguably now the limiting technology for application of genomics to cancer research. We made substantial progress in this area in the current funding period and now will expand these efforts with longer and more specialized workshops tailored to beginning and advanced applications, additional staff time for bioinformatics, and access to cancer-related pathway databases.