Analytical Chemistry Core

项目摘要/摘要 杜克大学超级基金研究中心(DUSRC)研究了早年暴露于 危险化学品和以后的生活后果。分析化学核心(ACC， 核心B)是为研究提供与超级基金有关的污染物的常规样本分析和监测 DUSRC内的项目。此外，ACC还提供识别转型的分析专业知识 这些超级基金污染物的产物和/或代谢物的高分辨质谱学 技巧。在过去的供资期间，行政协调会一直是刚果民主共和国的一个重要组成部分，并 产生了大量数据，以支持所有发改委项目的研究活动(超过2500个 截至2015年1月分析的样本)。定量分析主要集中在测定溴化物和 替代阻燃剂、有机磷农药(及其转化产物)和多环芳烃 各种痕量(ppb或更低)的生物和环境样品。除了例行程序之外 为支持DUSRC项目而开展的量化分析工作，行政协调会工作人员一直积极参与 在方法开发活动中，在项目中引入了新的分析物和样品类型。 实例包括生物固体和1，2-四溴双酚A及其脱溴产物的分析。 细胞培养液中的双(2，4，6-三溴苯氧基)乙烷。在应用高分辨率方面取得了进展 用于新污染物的非定向和效应导向分析的质谱学和其他方法 以及超级基金相关化学品在环境和生物介质中的转化产品。在这里，我们 提议继续和扩大行政协调会的活动，并将重点扩大到有针对性地分析 发育性毒物，包括有机(如多环芳烃、有机磷农药 和芳基有机磷酸酯、卤代芳香族和酚类以及替代阻燃剂)和 生物和环境样品中的无机污染物(如汞、砷和硒) 支持所有DUSRC项目。这将使用已建立的协议和现有的 与有经验的操作员进行仪器(如GC-MS和LC-MS/MS)。此外，我们还提出了一个 利用高分辨率质谱学提高非靶向和转化产物分析的作用 仪器与新的数据分析策略相结合。行政协调会将继续作为一种培训和 DUSRC内的分析化学咨询服务。最后，我们在分析方法方面的专业知识 发展将对新出现的污染物产生影响，这些污染物被确定为对 在SRC项目内进行调查。这些活动将得益于我们迄今在 开发、验证和应用环境和生物中的污染物分析方法 样本。

Project Summary/Abstract The Duke University Superfund Research Center (DUSRC) examines the problem of early life exposure to hazardous chemicals and later life consequences. The overall function of the Analytical Chemistry Core (ACC, Core B) is to provide routine sample analysis and monitoring of Superfund-relevant contaminants for Research Projects within the DUSRC. Furthermore the ACC provides analytical expertise in identifying transformation products and/or metabolites of these Superfund contaminants using high-resolution mass spectrometry techniques. The ACC has been an essential component of DUSRC over the past funding period, and has generated a large quantity of data in support of the research activities of all DUSRC Projects (Over 2,500 samples analyzed as of January, 2015). Quantitative analyses have centered on determining brominated and alternative flame retardants, organophosphate pesticides (and their transformation products), and PAHs in a variety of biological and environmental samples at trace (ppb or lower) levels. In addition to the routine quantitative analysis work performed in support of the DUSRC Projects, ACC staff have been actively involved in method development activities as new analytes and sample types have been introduced into the Projects. Examples include analysis of tetrabromobisphenol A and its debromination products in biosolids and 1,2- bis(2,4,6-tribromophenoxy)ethane in cell culture media. Progress has been made in applying high-resolution mass spectrometry and other methods for non-targeted and effects-directed analysis of novel contaminants and transformation products of Superfund-relevant chemicals in environmental and biological media. Here, we propose to continue and extend the activities of the ACC, with an expanded focus on targeted analysis of developmental toxicants including organic (e.g. polycyclic aromatic hydrocarbons, organophosphate pesticides and aryl organophosphate esters, halogenated aromatics and phenolics, and alternative flame retardants) and inorganic (e.g. mercury, arsenic, and selenium) contaminants in biological and environmental samples, in support of all DUSRC projects. This will be accomplished using established protocols and existing instrumentation (e.g. GC-MS and LC-MS/MS) with experienced operators. In addition, we propose an increased role for non-targeted and transformation product analysis using high resolution mass spectrometry instrumentation coupled to novel data analysis strategies. The ACC will continue to act as a training and consulting service for analytical chemistry within DUSRC. Finally, our expertise in analytical method development will be brought to bear on new and emerging contaminants identified as important for investigation within the SRC Projects. These activities will draw on our considerable success to date in developing, validating, and applying methods for contaminant analysis in environmental and biological samples.