An upright multiphoton microscope for biomedical research applications

项目摘要 在这项提案中，我们要求为购买多光子显微镜系统提供资金 位于加州大学贝克曼高级科学与技术研究所 伊利诺伊州。最先进的显微镜系统将使深层组织成像成为固定和 活组织，并为伊利诺伊大学的多名研究人员提供共享资源。 显微镜将配备扩展的红外激光，光遗传刺激 能力和易于移动的平台允许在体外和体内电生理学 实验。此外，显微镜将由贝克曼支持和管理 研究所显微镜套件，已经有专门的空间和全日制博士级别 管理仪器的工作人员。显微镜套件是一个完全由 贝克曼研究所，拥有在管理高端激光方面拥有丰富经验的受薪员工- 基于显微镜的系统。显微镜套件也有基础设施，以确保最大 美国国立卫生研究院资助用户对显微镜的使用。11个主要用户和1个次要用户拥有 他们的不同兴趣代表了贝克曼的跨学科性质 研究所。主要用户来自8个不同的部门，并使用多光子 显微镜将支持他们的NIH支持的研究，资金来自8个不同的 研究所(NCI、NIAID、NIBIB、NIDA、NIDCD、NIMH、NIGMS和NINDS)。主要用户有 对开发癌症诊断新技术平台的研究兴趣， 了解遗传性认知障碍的生物学基础，以便理解 无脊椎动物神经生物学，研究神经胶质-神经元相互作用和其他。因此， 多光子显微镜将支持各种不同领域的基础研究和翻译研究 学科，并支持在伊利诺伊州进行的NIH资助的研究。

Project Summary In this proposal, we request funding for the purchase of a multiphoton microscope system to be housed in the Beckman Institute for Advanced Science and Technology at the University of Illinois. The state-of-the-art microscopy system will permit deep-tissue imaging into fixed and live tissues and serve as a shared resource for multiple investigators at the University of Illinois. The microscope will be outfitted with an extended infrared laser, optogenetic stimulation capability and an easily moveable stage to permit in vitro as well as in vivo electrophysiology experimentation. In addition, the microscope will be supported and managed by the Beckman Institute Microscopy Suite, for which there is already dedicated space and full-time Ph.D.-level staff to manage the instrument. The microscopy suite is a unit that is fully supported by the Beckman Institute, with paid staff who have extensive experience in managing high-end laser- based microscopy systems. The Microscopy Suite also has infrastructure to ensure maximal utilization of the microscope by NIH-funded users. Eleven major users and one minor user have been identified, and their diverse interests represent the interdisciplinary nature of the Beckman Institute. The major users come from 8 different departments and use of a multiphoton microscope will support their NIH-supported research, with funding coming from 8 different institutes (NCI, NIAID, NIBIB, NIDA, NIDCD, NIMH, NIGMS and NINDS). The major users have research interests in developing novel technology platforms for cancer diagnosis, for understanding the biological underpinnings of inherited cognitive disorders, for understanding invertebrate neurobiology, studying glial-neuronal interactions and others. Therefore, the multiphoton microscope would support both basic and translational research across a variety of disciplines, and support ongoing NIH-funded research at Illinois.