Analytical Shared Resource Group

流式细胞仪共享资源(FCSR)成立于1985年，是UTHSCSA共享资源。 1991年，它被整合到癌症中心支持赠款(CCSG)中，作为癌症中心共享 资源。FCSR的使命是提供高通量、多维的细胞分析和细胞 为癌症治疗和研究中心(CTRC)成员使用最先进的设备进行分类。这个 FCSR由CTRC和UTHSCSA联合管理。FCSR在两个地点运作；漫长的校园 以及位于新建的南得克萨斯研究设施(STRF)的格里希校区，每个网站都在管理 一台细胞分选机和一台细胞分析仪。长长的校园设施位于800平方英尺的地方。在5.044Vand房间 拥有一辆FACSARia和一辆SORP LSRII。STRF场地位于1,306平方英尺。(251室)。它容纳了一个 MoFlo Astrios和FACSCalibur。FCSR服务包括多参数分析和排序 细胞亚群；细胞周期分析和分类；单细胞克隆；一氧化氮的测量， 氧过氧化和自由基、pH、钙离子通量和不同活性染料的通量；线粒体的研究 损伤；以及多个激活的半胱氨酸酶的测定。使用细胞仪微珠阵列(CBA) 技术，可以同时测量多种细胞因子的定量。FCSR积极提供 咨询服务包括实验设计、仪器培训、助学金和手稿写作 (包括编制数字、方法章节和预算)。FCSR人员包括本杰明·丹尼尔博士， FCSR主任(13年工作经验)。Vivienne Rebel博士，科学顾问(22年经验)和 Karia Gorena，BS，技术总监，主要仪器操作员(7年经验)。这支队伍 利用他们的专业知识以理论、仪器和技术支持的形式为用户提供培训 以符合成本效益的价格。在上一个获奖年度，FCSR服务支持了23项同行审查资助和20项 非同行评审资助的癌症中心成员，占共享资源总使用量的73%。在……里面 此外，在本授奖期间，FCSR服务贡献了48篇与癌症有关的手稿 (2008-2013)。

The Flow Cytometry Shared Resource (FCSR) was established in 1985 as a UTHSCSA Shared Resource. In 1991, it was integrated into the Cancer Center Support Grant (CCSG) as a Cancer Center Shared Resource. The mission of the FCSR is to deliver high-throughput, multi-dimensional cell analysis and cell sorting using state-of-the-art equipment for Cancer Therapy & Research Center (CTRC) members. The FCSR jointly managed by CTRC and UTHSCSA. The FCSR operates at two locations; the Long campus and the Greehey campus in the newly built South Texas Research Facility (STRF ), with each.site managing one cell sorter and one cell analyzer. The Long campus facility is located in 800 sq.ft. in room 5.044Vand houses a FACSAria and a SORP LSRII. The STRF site is located in 1,306sq.ft. (room 251). It houses a MoFlo Astrios and a FACSCalibur. FCSR services include multiparameter analysis and sorting of subpopulations of cells; cell cycle analysis and sorting; single cell cloning; measurements of nitric oxide, oxygen peroxide and free radicals, pH, Ca++ fluxes and fluxes of different vital dyes; study of mitochondrial damage; and determination of multiple activated caspases. Using the cytometry bead array (CBA) technology, quantification of multiple cytokines can be measured simultaneously. The FCSR actively offers consulting services ranging from experimental design, instrument training, and grant and manuscript writing (including preparing figures, method sections and budgets). FCSR personnel includes Dr. Benjamin Daniel, FCSR Director (13 years of experience). Dr. Vivienne Rebel, Scientific Advisor, (22 years of experience) and Karia Gorena, BS, Technical Director, the primary instrument operator (7 years of experience). This team uses their expertise to provide training to users in the forms of theory, on instrument, and technical support at cost-effective rates. During the last award year, FCSR services supported 23 peer-review funded and 20 non peer-review funded cancer center members, comprising 73% of the total shared resource usage. In addition, the FCSR services contributed to 48 cancer-related manuscripts during the current award period (2008-2013).