National Swine Resource and Research Center 1

摘要（总体） 本申请的总体目标是继续和扩大国家猪资源的运作， 研究中心（NSRRC）。NSRRC已经运营了14年，它已经开发了 基础设施需要协助猪为基础的研究跨多个学科，包括 异种移植NSRRC通过基因工程开发了人类疾病的新模型， 它招募了其他人创造的模型，并分发了专业知识，细胞，组织，器官和猪， 美国各地的调查人员。由于猪的基因工程需要专门的设施， 和专业知识，NSRRC通过使用最新的 基因编辑技术，创造新的基因工程猪模型。NSRRC还提供 通过与个人互动、出版手稿和主办讲习班进行培训和教育 以及推广使用猪研究人类疾病的会议。国家核安全研究委员会有最先进的 建筑物具有高度生物安全性，以饲养无特定病原体的动物。具体目标 即将到来的资助期如下： 1.经营国家猪研究和资源中心。NSRRC的职能将继续 包括：a）引进现有人类健康和疾病的猪模型; B）重新衍生猪， 消除特定病原体，并进行健康监测，以确保维持无特定病原体 c）冷冻保存和储存配子、胚胎和体细胞，以防止将来的损失。 有价值的模型; d）分配专门知识、试剂、细胞、组织、器官和无病原体猪;以及e） 使用最先进的技术来创造生物医学所需的新的基因工程猪 研究社区。严格的质量控制将确保只有高质量的基因被分发。的 NSRRC还将作为与猪研究相关的培训和教育活动的场所 模型 2.进行创新研究，这将有利于NSRRC和生物医学研究界。 应用研究项目旨在改进：a）猪生殖细胞的冷冻保存， 组织，B）生产基因工程猪的方法，c） 猪健康监测，以及d）正确的猪大小，以更广泛地采用猪模型。

Abstract (Overall) The overall goal of this application is to continue and expand operation of the National Swine Resource and Research Center (NSRRC). The NSRRC is in its 14th year of operation and it has developed the infrastructure needed to assist swine-based research across multiple disciplines, including xenotransplantation. The NSRRC has developed new models of human disease by genetic engineering, it has recruited models created by others, and it has distributed expertise, cells, tissues, organs and pigs to investigators throughout the United States. Since genetic engineering of pigs requires specialized facilities and expertise, the NSRRC provides invaluable services to the research community by using the latest in gene editing technology to create new genetically-engineered swine models. The NSRRC also provides training and education by interacting with individuals, publishing manuscripts and sponsoring workshops and meetings that promote the use of pigs for studying human diseases. The NSRRC has a state-of-the-art building with high biosecurity to house animals free of specific pathogens. The Specific Aims for the upcoming grant period are as follows: 1. To operate the National Swine Research and Resource Center. Functions of the NSRRC will continue to include: a) importation of existing swine models of human health and disease; b) rederivation of pigs to eliminate specific pathogens, and health monitoring to assure maintenance of a specific pathogen-free status; c) cryopreservation and storage of gametes, embryos, and somatic cells to prevent future loss of valuable models; d) distribution of expertise, reagents, cells, tissues, organs, and pathogen-free pigs; and e) use state of the art technology for the creation of new genetically-engineered pigs needed by the biomedical research community. Rigorous quality control will ensure that only high quality genetics are distributed. The NSRRC will also serve as a site for training and educational activities related to research employing swine models. 2. To perform innovative research that will benefit the NSRRC and the biomedical research community. Applied research projects are aimed at improving: a) the cryopreservation of pig reproductive cells and tissues, b) methods for the production of genetically-engineered pigs, c) state-of-the-art testing methods for swine health surveillance, and d) right-size the pig for greater adoption of pig models.