200keV, Energy Filtered, Intermediate-High Voltage Transmission Electron Microscope(IVEM)"

项目总结/摘要 该申请概述了我们的资金申请，以采购一个新的200 keV，能量过滤，中高 电压透射电子显微镜（IVEM）（Thermo-Fisher/FEI Talos F200 C G2）。该仪器将 完全独特的拉霍亚，加州研究梅萨（和它的许多研究组织），并纳入 生产使用先进的（和互补的）方法，用于启用能量过滤器的多标签传输 电子显微术（例如，ColorEM）和半- 厚环氧树脂包埋的细胞和组织样本（非冷冻）。采购该显微镜将解决 两个迫切的需求：1）它将直接支持这些成像方法/技术的需求增长， 当地和全国分布的NIH研究人员，目前国家显微镜中心的用户， 成像研究（NCMIR）; 2）它将提供生产访问以前只有尖端的方法 可在高端，专门的平台上使用，以实现ColorEM等方法的开发（实现 NIH的目标是降低使用障碍，促进更广泛的传播和影响）。因此，它也将 推动这些关键技术的民主化。指定的新仪器将于 与它将取代的仪器相比，成本显著降低，该仪器是一台有20年历史的定制JEOL 3200 IVEM， 很快就不被JEOL维护了。新工具还简化了安装，空间要求更低， 允许更多的NIH研究人员采用这种方法。平台将自动化并设置- 为社区提供方便，并得到电子显微镜，标本 准备和数据处理，分析和可视化。它的环境还将包括（并使直接 使用）圣地亚哥超级计算机的新型高性能计算和数据存储基础设施 中心及其附属的国家研究平台（NRP）。

PROJECT SUMMARY / ABSTRACT This application outlines our request for funding to procure a new 200keV, energy filtered, intermediate-high voltage transmission electron microscope (IVEM) (Thermo-Fisher/FEI Talos F200C G2). This instrument will be completely unique to the La Jolla, CA research Mesa (and it’s many research organizations) and bring into production use cutting edge (and complementary) methods for energy filter enabled, multiple label transmission electron microscopy (e.g., ColorEM) and high throughput 3D electron microscopic tomography (3D EM) of semi- thick epoxy-embedded samples of cells and tissues (non-Cryo). Procurement of this microscope will address two pressing needs: 1) it will directly support growing demand for these imaging methods/technologies by both local and Nationally distributed NIH investigators, currently users of the National Center for Microscopy and Imaging Research (NCMIR); and 2) it will provide production access to cutting-edge methods previously only available on high-end, specialized platforms built to enable development of methods like ColorEM, (fulfilling a goal of NIH to lower barriers to the use and to promote broader dissemination and impact). As such, it will also propel the democratization of these key technologies. The specified new instrument will be procured at a significantly lower cost, compared to the instrument it will replace, a 20 year old custom-built JEOL 3200 IVEM, soon not maintainable by JEOL. The new tool also simplifies installation, with more modest room requirements, allowing adoption of the methods it enables by more NIH researchers. The platform will be automated and set- up for easy access by the community and be supported by a team of experts in electron microscopy, specimen preparation and data processing, analysis, and visualization. Its environment will also include (and make direct use) of novel high-performance computing and data storage infrastructure of the San Diego Supercomputer Center and its affiliated National Research Platform (NRP).