Rodent Cancer Models Core

摘要-啮齿动物癌症模型共享资源 啮齿动物癌症模型共享资源(RCM)为杜克癌症研究所(DCI)成员提供了完整的 癌症生物学研究的服务途径--从啮齿动物模型的建立到实验 化疗药物。为了实现这一目标，DCI的RCM结合了两项主要服务：癌症中心 隔离设施(CCIF)和啮齿动物基因工程服务团队(RGEST)。CCIF是一个3万平方英尺的 脚，独立的啮齿动物屏障设施，位于杜克大学生物医学园区的中心，由公爵运营 实验动物资源部(DLAR)，致力于癌症研究。CCIF实体工厂 支持生物安全2级(BSL2)、重组DNA、化疗药物等危险材料的工作 以及维持免疫抑制(近交系裸鼠和近交系NOD SCID Gamma(NSG)) 小鼠)和无特定病原体(SPF)的啮齿动物。在CCIF和附近设施中占用空间 生产定制设计、基因改变、转基因和基因靶向的小鼠。RCM人员协助DCI 在育种服务、兽医或诊断服务、动物和动物方面需要协助的调查人员 细胞系的实验方案的制定、接种或测试，以及进行安全和 高效的癌症生物学研究。CCIF还为CCSG提供设备和鼠房- 支持光学分子成像和分析(OMIA)共享资源。最近，重大投资 由杜克医学院提供的数据支持对CCIF和RGEST设备进行升级。 2018年，RCM向78名调查人员提供了服务，其中61%是DCI成员和世卫组织 占总使用量的94%，代表了所有8个DCI研究项目。使用此共享资源 在项目期间，发展中心成员为332份发展合作组织出版物作出了贡献，其中103份影响较大 期刊(影响系数和GT；9)，展示了RCM服务的价值。当前和长期目标 的RCM正在继续提供高质量的屏障设施服务，低成本、高质量 使用尖端方法的免疫受损和SPF小鼠以及基因工程动物模型， 尽可能改进和提升服务，并向DCI成员提供关键的专业知识和服务 研究癌症生物学，开发治疗或诊断人类癌症的新药物。

ABSTRACT – RODENT CANCER MODELS SHARED RESOURCE The Rodent Cancer Models Shared Resource (RCM) provides Duke Cancer Institute (DCI) members a full service approach to the investigation of cancer biology, from the creation of rodent models through the testing of chemotherapeutics. To achieve that goal DCI’s RCM combined two primary services: the Cancer Center Isolation Facility (CCIF) and the Rodent Genetic Engineering Services Team (RGEST). CCIF is a 30,000 square foot, stand-alone rodent barrier facility located in the heart of the Duke biomedical campus operated by the Duke Division of Laboratory Animal Resources (DLAR) and dedicated to cancer research. The CCIF physical plant supports work with hazardous materials at biosafety level 2 (BSL2), recombinant DNA, chemotherapeutics, as well as the maintenance of immunosuppressed (outbred athymic nude mice and inbred Nod SCID gamma (NSG) mice) and specific pathogen-free (SPF) rodents. Occupying space in CCIF and in nearby facities, RGEST produces custom designed, genetically altered, transgenic and gene-targeted mice. RCM personnel assist DCI investigators who require assistance with breeding services, veterinary or diagnostic services, animal and experimental protocol development, inoculation or testing of cell lines, and guidance for conducting safe and efficient cancer biology research. CCIF also provides equipment and rodent housing space for the CCSG- supported Optical Molecular Imaging and Analysis (OMIA) shared resource. Recently, significant investments by the Duke School of Medicine have enabled upgrades to CCIF and RGEST equipment. In 2018, the RCM provided services to 78 investigators, 61% of whom were DCI members and who accounted for 94% of total usage and represented all 8 DCI Research Programs. Use of this shared resource by DCI Members contributed to 332 DCI publications over the project period, 103 of which were in high impact journals (Impact Factor>9), demonstrating the value of RCM services. The immediate and long-term objectives of the RCM are to continue to provide high-quality barrier facility services, low-cost, high-quality immunocompromised and SPF mice and genetically engineered animal models using cutting edge approaches, improve and upgrade services when possible, and to provide key expertise and services to DCI members studying cancer biology and developing new agents for treating or diagnosing human cancers.