High-resolution reflective microscope

该SBIR项目的目标是开发和商业化一种全新类型的全反射 显微镜(ARM)，从紫外线(UV)到红外(IR)的全光谱应用，无任何遮挡。在……里面 第一阶段，Light Research Inc.(LRI)将通过设计、原型制作和表征 大数值孔径(NA)反射物镜和管状透镜。 由于材料的色散和吸收，目前的折射显微镜有许多局限性： 色差、吞吐量、群延迟色散(GDD)和窄的工作光谱范围。而当 成功地解决了色差和群延迟色散的限制，传统的 解决方案-轴上反射物镜-由于中央遮挡，仍有其他一些主要问题，防止 它在生物研究中被广泛采用：低通量、低对比度和低图像质量。 为了解决当前最先进的折射和反射显微镜中的所有局限性，一种全新的 而真正没有遮挡的手臂将被开发出来。该臂的关键创新之处在于离轴配置 使用自由曲面。具有自由曲面的离轴、非遮挡物镜将解决所有相关问题 相对于传统的反光物镜，具有以下独特的关键特性：高图像对比度、高图像 高质量、高光效、高吞吐量、零色差和低GDD。LRI将许可快照- 亚利桑那大学(UA)组装技术开发和商业化无对准Off-Off 轴，非遮挡反射物镜。 第一阶段项目的目标将通过两个目标实现：(1)设计ARM，和(2)原型和 角色化手臂。随着ARM在第一阶段项目中获得的知识和成功的演示，在 Ii LRI将开发和商业化一系列具有不同Nas和管状透镜的反射物镜 不同的焦距。 凭借独特的从UV到IR的无遮挡的高性能成像能力，真正的ARM将 不仅简化了目前多模和宽带成像系统的配置，而且能够 研究人员将通过获得新的多模式和 宽带数据在全频谱中具有更高的吞吐量和速度，并且正在开发新的成像技术 与当前的显微镜平台配合使用成为可能。

The goal of this SBIR project is to develop and commercialize a fundamentally new type of all-reflective microscope (ARM) without any obscuration for full spectrum applications from ultraviolet (UV) to infrared (IR). In Phase I stage, Light Research Inc (LRI) will demonstrate ARM by designing, prototyping, and characterizing a high numerical aperture (NA) reflective objective and tubing lens. Current refractive microscope has a number of limitations due to the material dispersion and absorption: chromatic aberrations, throughput, group delay dispersion (GDD), and narrow working spectral range. While successfully addressing the limitations on chromatic aberration and group delay dispersion, the traditional solution - on-axis reflective objective - still has some other major issues due to the central obscuration, preventing it from being widely adopted in biological research: low throughput, low contrast, and low image quality. To address all limitations in the current state-of-the-art refractive and reflective microscope, a fundamentally new and true ARM without obscuration will be developed. The key innovation of this ARM is the off-axis configuration with freeform surfaces. Off-axis, non-obscuration objective with freeform surfaces will address all issues related to traditional reflective objective, and has the following unique key properties: high image contrast, high image quality, high light efficiency, high throughput, zero chromatic aberration, and low GDD. LRI will license the snap- on assembling technology from University of Arizona (UA) to develop and commercialize the alignment-free off- axis, non-obscuration reflective objective. The goal of the Phase I project will be achieved through two aims: (1) design ARM, and (2) prototype and characterize ARM. With the gained knowledge and successful demonstration of ARM in Phase I project, in Phase II LRI will develop and commercialize a series of reflective objectives with different NAs and tube lenses with different focal lengths. With the unique capability of high-performance imaging from UV to IR without any obscuration, the true ARM will not only simplify the configurations of the current multimodal and broadband imaging systems, but also enable investigators to advance the biological and biomedical research through acquiring new multimodal and broadband data with higher throughput and speed in full spectrum, and developing new imaging techniques not possible with current microscope platform.