THE PRIMATE EMBRYO GENE EXPRESSION RESOURCE

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目的：灵长类动物胚胎基因表达资源允许使用恒河猴作为主要模式生物快速和成本效益的灵长类动物卵母细胞和植入前胚胎的基因表达研究。PREGER包含超过160个信息样本，代表GV期卵母细胞、MII期卵母细胞和1-细胞至孵化囊胚期的胚胎，使用体外或体内成熟、受精和培养的不同组合获得。这一广泛而有价值的样本集允许分析与理解基本灵长类动物胚胎学相关的基因表达，以及使用灵长类动物模型临床程序对胚胎质量的潜在影响。PREGER中用于表达分析的斑点印迹、cDNA文库、探针和其他工具可以分发给其他科学家，为灵长类动物胚胎的分子研究提供了前所未有的机会。文库可用于无数其他目的，例如文库筛选和文库消减。我们建议维护资源，继续向科学界分发这些材料和分子工具，维护PREGER网站，将资源扩展到包括狒狒样本，以便可以评估灵长类物种中观察结果的一般性，并纳入人类和恒河猴ES细胞系和恒河猴内细胞团细胞样本，以评估人类ES细胞系的发育潜力。我们还建议应用一种新的方法，我们已经开发出与我们的扩增文库兼容的抑制消减杂交，以确定新的发育调控基因，这些基因与早期灵长类动物胚胎发生相关，并将有助于辅助生殖方法的改进。这将促进基础研究，并可能支持新的临床方法，例如，评估胚胎质量。拟议的活动将立即维持和扩大资源，产生有关灵长类动物胚胎的新的和基本的基本信息，并产生可能在临床实践中有用的新的分子方法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: The Primate Embryo Gene Expression Resource permits the rapid and cost-effective study of gene expression in primate oocytes and preimplantation embryos using the rhesus monkey as the primary model organism. PREGER contains over 160 informative samples representing GV-stage oocytes, MIIstage oocytes, and embryos at the 1-cell through hatched blastocyst stages, obtained using different combinations of in vitro or in vivo maturation, fertilization, and culture. This extensive and valuable sample set permits analyses of gene expression related to understanding basic primate embryology, as well as potential effects of clinical procedures on embryo quality using a primate model. Dot blots for expression analysis, cDNA libraries, probes, and other tools in PREGER can be distributed to other scientists, providing unprecedented opportunities for molecular studies in primate embryos. The libraries can be employed for a myriad of other purposes, such as library screening and library subtraction. We propose to maintain the resource, continue to distribute these materials and molecular tools to the scientific community, maintain the PREGER website, extend the resource to encompass samples from baboon in order that the generality of observations among primate species may be evaluated, and incorporate samples of human and rhesus ES lines and rhesus inner cell mass cells for evaluating the developmental potency of human ES lines. We also propose to apply a novel method for suppression subtraction hybridization that we have developed to be compatible with our amplified libraries, in order to identify novel developmentally regulated genes that are relevant to early primate embryogenesis and that will assist improvements in assisted reproduction methods. This will facilitate basic research, and may support novel clinical approaches, e.g., in the evaluation of embryo quality. The proposed activities will at once maintain and expand the resource, produce new and fundamental basic information about primate embryos, and produce novel molecular approaches that may be useful in clinical practice.