STRUCTURAL ANALYSIS OF SPINACH ACYL CARRIER PROTEIN

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 实验室以前的工作表明，随着附着在ACP上的脂肪酸从10个碳延长到18个碳，ACP内部30到40个残基的运动增加，表现为15N HSQC峰的加宽以及15N T2弛豫的增加。本研究的目标是仔细表征具有15、16和17个碳脂肪酸取代基的ACP的松弛行为，因为这些行为可能表明ACP的稳定折叠和残基30-40的不稳定之间的过渡。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Previous work in the lab has shown that as the fatty acid attached to ACP elongates from 10 carbons to 18 carbons, there is an increase in the motions of residues 30 through 40 within ACP, as shown by broadening of 15N HSQC peaks as well as increasing 15N T2 relaxation. The goal of this study is to carefully characterize the relaxation behavior of ACP with 15, 16, and 17 carbon fatty acid substituents, as these are likely to demonstrate the transition between a stable fold for ACP and the destabilization of residues 30-40.