TYPE II SECRETION SYSTEM FROM VIBRIO CHOLERAE AND RELATED PATHOGENS

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 革兰氏阴性细菌已经进化出许多蛋白质机器，用于通过细胞膜输出小分子和整个蛋白质。II型分泌系统(T2SS)使这些细菌能够通过外膜从周质中输出折叠蛋白。T2SS由12-15个蛋白质组成，从外膜的蛋白质传导孔通过一系列周质蛋白延伸到内膜亚复合体和胞浆中的一个调节ATPase。人类病原体霍乱弧菌使用这样的系统出口水解酶和霍乱毒素(CT)，霍乱毒素是毁灭性疾病霍乱的主要病原体。在产肠毒素大肠杆菌(ETEC)中也发现了类似的系统，它会分泌与CT相近的不耐热肠毒素(LT)。ETEC是发展中国家儿童腹泻、脱水和死亡的主要原因。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Gram negative bacteria have evolved a number of protein machineries for the export of small molecules and entire proteins through the cell envelope. The Type II Secretion System (T2SS) enables these bacteria to export folded proteins from the periplasm through the outer membrane. The T2SS consists of 12-15 proteins, which span from the protein conducting pore in the outer membrane via a set of periplasmic proteins to the inner membrane subcomplex and a regulating ATPase in the cytosol. The human pathogen Vibrio cholerae uses such a system for the export of hydrolytic enzymes and cholera toxin (CT), the major causative agent of the devastating disease cholera. A similar system is found in Enterotoxigenic E. coli (ETEC) which secretes heat-labile enterotoxin (LT), a close homolog of CT. ETEC is a major cause of childrens diarrhea, dehydration and death in developing countries.