IMAGING CORE

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 这一核心将专注于鉴定骨髓细胞移植后的细胞性质。供体细胞及其特定谱系将通过共聚焦和去卷积荧光显微镜进行确定和共定位。通过绿色荧光蛋白、β-半乳糖苷酶的表达或男性DNA的存在，将在组织切片或细胞悬液中追踪捐赠者细胞。已鉴定的供体细胞的谱系将通过标准的形态、地理位置和免疫组织化学来确定。供体和谱系标记的共定位将使用共聚焦或去卷积荧光显微镜进行确认。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This Core will focus on identifying the nature of cells after marrow cell transplantation. Donor cells and their specific lineages will be ascertained and colocalized by confocal and deconvolution fluorescent microscopy. Donor cells will be tracked in tissue sections or cell suspensions by expression of GFP, beta-galactosidase or the presence of male DNA. The lineage of identified donor cells will be ascertained by standard morphology, geographical location and immunohistochemistry. Colocalization of donor and lineage markers will be confirmed using confocal or deconvolution fluorescent microscopy.