Analytic and Preparative Cytometry Facility

流式细胞分析仪核心（APFCC）的设计目的是提高功率， 风湿病核心中心（RDCC）研究人员的研究生产力，通过提供国家的， 以合理的成本进行细胞分析和纯化的流式细胞术。为了实现这一目标， Core提供流式细胞仪应用所需的设备、服务和专业知识， 与风湿性疾病前沿研究相关的技术。我们使用了全国同行评审的 联邦仪器赠款和UAB资源，以获得顶级设备， RDCC调查人员的广泛应用。这包括20个检测器、4个激光分析细胞仪 （2006年获得），14个探测器，3个激光器，一个分选增强型全数字分选细胞仪（2006年升级），一个6- 检测器、装载器配备的分析器和5检测器分析器。此外，核心还提供设备， 辅助技术，如自动样品制备（2005年获得），荧光分光光度法， 自动磁浓缩（2005年获得）。我们开发了新技术， 基本的样本分析，结果是APFCC已经为120多个独立资助的UAB提供服务 调查员，并为许多出版物做出了贡献。我们的核心目标是继续努力扩大 和教育，提醒RDCC调查人员注意流动技术的潜力，以增加他们的权力， 研究，服务，以提高效率和保持最高的质量标准，以及 应用开发，继续为RDCC研究带来新技术和协议 社区为了实现这些目标，需要资金来支持外联、教育、质量控制 核心主任和核心管理人员的发展活动，部分抵消了一个 服务合同，以保持不间断地访问这一重要技术。科学咨询委员会， 它结合了知识渊博的用户和参与UAB高级研究职位的人员，UAB是一个 外部顾问，他对新设备和应用程序了如指掌，并与 更大的UAB机构将提供反馈并推动进一步发展。通过这些努力，我们将 维持流式细胞术作为风湿性疾病最先进研究的基本、重要工具， UAB。

The Analytic and Preparative Flow Cytometry Core (APFCC) is designed to enhance the power and productivity of the research of Rheumatic Disease Core Center (RDCC) investigators by providing state-ofthe- art flow cytometry for cell analyses and purification at a reasonable cost. To accomplish this goal, the Core provides the equipment, service and expertise necessary for the application of flow cytometry and related technologies to cutting edge research in the rheumatic diseases. We have used nationally peerreviewed federal instrumentation grants and UAB resources to obtain top-of-the-line equipment that provides a wide range of applications for RDCC investigators. This includes a 20-detector, 4-laser analytic cytometer (obtained 2006), a 14-detector, 3-laser, a sort enhanced all digital sorting cytometer (upgraded 2006), a 6- detector, loader equipped analyzer, and a 5-detector analyzer. In addition, the core provides equipment for ancillary techniques such as automated sample preparation (acquired 2005), spectrofluorimetry, and automated magnetic enrichment (acquired 2005). We have developed new techniques as well as provided basic sample analysis, with the result that the APFCC has served over 120 independently funded UAB investigators and contributed to many publications. Our goals for the core are to continue efforts at outreach and education, to alert RDCC investigators to the potential for flow technology to increase the power of their research, service, to improve efficiency and to maintain the highest possible standards of quality, and application development, to continue to bring new technologies and protocols to the RDCC research community. To achieve these goals, funds are requested to support outreach, educational, quality control and development activities of the Core Director and Core Manager and partially offset the costs of one service contract to maintain uninterrupted access to this vital technology. The-Scientific Advisory Committee, which combines both knowledgeable users and those involved in senior research positions at UAB, an external advisory, who is well informed about newer equipment and applications, and an integration with the larger UAB institution will provide feedback and drive further development. With these efforts, we will maintain flow cytometry as a fundamental, vital tool for state-of-the-art research in the rheumatic diseases at UAB.