Transgenic Gene Knockout

通过转基因和基因敲除技术建立的动物模型在评估基因功能方面具有不可估量的价值 在体内以及在确定某些基因如何调节细胞生长和分化以及如何改变 这些基因会导致癌症。在这个设施中对小鼠进行基因改造，要么是通过注射原核 DNA进入受精卵，通过将逆转录病毒注射到受精卵的周围线空间，或通过注射 突变的胚胎干细胞(ES细胞)进入囊胚。此共享资源的目标是提供 在最短的时间内将转基因小鼠提供给癌症中心的首席研究人员--最具成本效益 道路。此外，这些小鼠品系的可获得性极大地刺激了 圣犹大的调查人员。

Animal models created by transgenic and gene knockout technology are invaluable in evaluating gene function in vivo and in determining how certain genes regulate cell growth and differentiation and how alterations in these genes contribute to cancer. Mice are genetically modified in this facility either by pronucleus injection of DNA into fertilized eggs, by injection of retroviruses into the perivitteline space of fertilized eggs, or by injection of mutated embryonic stem cells (ES-cells) into blastocysts. The objective of this shared resource is to provide genetically modified mice to principal Investigators within the Cancer Center in the most time- and costeffective way. In addition, the availability of these mouse strains greatly stimulates interactions among investigators at St Jude.