Establishment of a SPF Rhesus Macaque Colony

描述（申请人提供）：该项目的目标是提高国家生产无特定病原体（SPF）猕猴的能力，用于艾滋病相关研究，方法是维持一个印度原产恒河猴的生产群体，这些恒河猴对疱疹B病毒、SIV、SRV和STLV-1都是SPF，并通过提供相对于MHC基因型表征的动物来满足近期和长期的特定研究需要。具体目标如下：1.通过评估和移除阳性动物的详细计划，向NIH资助的研究者提供以下动物数量：06年60只，07年60只，08年70只，09年70只，10年70只。殖民地将扩大到约430只动物的稳定种群，其中约有140只雌性饲养者，20只雄性饲养者，以及275只婴儿和青少年，每年可持续收获约70只动物用于研究; 2.通过向研究者提供具有以下Mamu等位基因特征的动物：Mamu-A*01、-A*02、-A*11、-B*01、-6 Mamu 03、-B*04和-B *17，最大限度地提高动物的研究价值。3.通过a）建立、管理和利用用于殖民地遗传管理的谱系数据库，使菌落生产的长期效率最大化。在前5年通过NIH/NCRR的其他资助开发的20个高度信息化的微卫星多态性小组，沿着人口统计学记录以建立该数据库，将用于建立和验证谱系信息，管理育种以避免近亲繁殖和保持遗传变异性，同时通过利用死亡率、发病率、和繁殖力的问题

DESCRIPTION (provided by applicant): The goals of this project are to increase the nation's capacity to produce pedigreed specific pathogen free (SPF) Macaca mulatta for AIDS-related research by maintaining a production colony of Indian-origin rhesus monkeys that are SPF for herpes B virus, SIV, SRV, and STLV-1, and to fulfill specific research needs in the immediate and long-term future by providing animals characterized relative to MHC genotype. The specific aims are as follows: 1. Through a detailed program of assessment and removal of positive animals, to provide the following numbers of animals to NIH-funded investigators: 60 in Year 06, 60 in Year 07, 70 in Year 08, 70 in Year 09 and 70 in Year 10. The colony will expand to a steady-state population of around 430 animals, with approximately 140 breeder females, 20 breeder males, and 275 infants and juveniles, enabling a continuing annual harvest of approximately 70 animals for research; 2. To maximize the value of the animals for research purposes by offering to investigators animals that are characterized relative to the following Mamu alleles: Mamu-A*01, -A*02, -A*11, -B*01, -6*03, -B*04, and-B*17.; 3. To maximize the long term efficiency of colony production by a) establishing, managing, and utilizing a pedigree database for genetic management of the colony. The panel of 20 highly informative microsatellite polymorphisms developed in the first 5 years through other funding from NIH/NCRR, along with demographic records to establish this database, will be used to establish and verify pedigree information, manage breeding to avoid inbreeding and maintain genetic variability, while focusing on the primary goal of maximizing production and harvest schedules by utilizing data on mortality, morbidity, and fertility in the colony.