ANALYSIS OF ACUTE SIVAGM INFECTION OF AFRICAN GREEN MONKEYS AND PIGTAIL MACAQUES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者的研究机构。 在自然感染猿猴免疫缺陷病毒（SIV）的过程中，非洲绿色猴（AGM）不会发展为艾滋病，尽管高病毒载量持续存在。猪尾猕猴的SIVagm感染确实会导致AIDS，其特征是CD 4 + T细胞耗竭和机会性感染。 在目前的研究中，我们正在比较猪尾猕猴和非洲绿色猴中SIVagm.Sab92018的感染。据认为，先天性促炎与抗炎反应的平衡可能有助于区分致病性和非致病性SIVagm和其他慢病毒感染。对于这两个物种，我们正在使用我们的猕猴微阵列进行转录分析，比较感染时间过程中每只动物多个组织的基因表达变化。其他参数包括病毒载量测定、细胞计数、细胞因子血浆水平、抗体应答、表型变化、T细胞应答（细胞因子分泌和增殖）等。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. During natural infection with the Simian immunodeficiency virus (SIV), African green monkeys (AGM) do not develop AIDS though high viral loads are persistent. SIVagm infection in Pig-tailed macaques does lead to AIDS, characterized by CD4+ T-cell depletion and opportunistic infections. In the current study, we are comparing infection of SIVagm.Sab92018 in Pig-tailed macaques and African green monkeys. It is thought that the balance of innate pro- vs. anti-inflammatory responses may contribute to the distinction between pathogenic and non-pathogenic SIVagm and other lentiviral infections. For both species, we are performing transcriptional profiling using our macaque microarrays comparing gene expression changes in each animal for multiple tissues over the infection time course. Additional parameters include viral load determination, cell counts, cytokine plasma levels, antibody response, phenotypic changes, T cell responses (cytokine secretion and proliferation), etc.