RHBMP-2 EFFECT ON BMD IN AN OSTEOPOROTIC BABOON VERTEBRAE + PROXIMAL FEMUR

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 本研究的目的是评估骨诱导剂rhBMP-2是否能够改善骨质疏松狒狒椎体和股骨的骨量，从而降低骨脆性和骨折风险。我们建议给动物注射含有重组人骨形态发生蛋白-2的固体POE颗粒。手术过程将非常类似于椎体成形术，即使用插管和荧光镜识别椎体上的椎弓根，然后穿透椎弓根，注射POE/rhBMP-2颗粒。入口的缺损处将用骨蜡封闭。同样的方法也可以用于灵长类的股骨近端。作为这项研究的结果，我们预计将观察到椎体和股骨近端的骨量增加。每个治疗组将检查DEXA、MicroCT和组织学参数。这个项目可以解决日益增长的治疗需求，这种疗法可以预防高危人群的骨质疏松骨折。目前旨在增加骨矿密度的系统疗法并不能消除由于骨质疏松而导致的大约75万个椎体骨折。骨形态发生蛋白，如重组人骨形态发生蛋白-2，已知的局部骨形成的记录，我们假设这种蛋白的局部输送将在快速增强局部骨结构方面取得成功。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The purpose of this study is to evaluate whether rhBMP-2, a bone inducing agent, is capable of improving bone mass, thereby reducing bone fragility and fracture risk, in vertebral bodies and femurs of osteoporotic baboons. We propose injecting the animals with a solid POE pellet containing rhBMP-2. The surgical procedure would be very similar to a vertebroplasty surgery, where a cannula and a fluoroscope would be used to identify the pedicle on the vertebral body, the pedicle would then be pierced, and the POE/rhBMP-2 pellet injected. The entrance defect would be sealed with bone wax. The same approach can be utilized in the proximal femur of the primate. As a result of the study we would expect to observe an increased bone mass of the vertebral body and the proximal femur. DEXA, microCT and histology will be the parameters that will be examined per each treatment group. This project could address the growing need for a therapy that could prevent osteoporotic factures in high risk individuals. Current systemic therapies aimed at increasing bone mineral density do not eliminate the estimated 750,000 vertebral factures that occur as a result of osteoporosis. Bone morphogenetic proteins such as rhBMP-2 have a known record of local bone formation and we hypothesize that local delivery of this protein will be successful in rapidly enhancing local bone structure.