ENDOCRINE CORE SERVICES

这个子项目是许多利用 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 目标：内分泌核心已经为22位科学家提供了检测，并收取了大约166，000美元。 我们的检测包括对血浆、粪便、尿液和唾液中类固醇激素的常规分析，以及其他地方无法提供的独特分析。 同样，它的检测开发是针对提供由我们的教师和合作者使用，但不容易提供给他们的检测。 在过去的一年里，内分泌核心已经扩大了其服务范围，包括代谢激素和载体蛋白。 我们的目的是为治疗、研究服务和个体研究者要求的所有检测提供内部服务。一些新的检测方法正在ACS-180自动化学发光平台上进行格式化。 猕猴甲状腺面板以及猴绒毛膜促性腺激素（mCG）测定现在可用。 新的基于细胞的生物测定现在提供了循环生物可利用的雌激素、雄激素和促黄体激素的直接测量。目前正在开发一元胺的高效液相色谱电磁检测法。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: The Endocrine Core has provided assays for twenty-two scientists, and recharged approximately $166,000. Our assays include routine analysis of steroid hormones in plasma, feces, urine and saliva and unique analyses that are not available elsewhere. Similarly, its assay development is directed towards providing assays that are used by our faculty and collaborators but are not readily available to them. During the past year the Endocrine Core has extended its service to include metabolic hormones and carrier proteins. It is our intent to provide in-house service for all assays requested through Therapeutics, Research Services and by individual investigators. Some of the new assays are being formatted on the ACS-180 automated chemiluminescence platform. A macaque thyroid panel as well as monkey chorionic gonadotropin (mCG) assays are now available. New cell based bioassays now provide direct measurements of circulating bioavailable estrogens, androgens and luteinizing hormone. HPLC with electromagnetic detection is being developed for assay of monoamines.