Midcareer Investigator Award in Patient-Oriented Research (K24)

描述（由申请人提供）：目标。近期和长期目标是：1）成为与肝病相关的转化，临床和基础研究的领导者; 2）培养下一代从事胃肠道和肝脏疾病的医生科学家。1我致力于加强医学生，居民，研究员和初级教师在研究的教育培训和促进以病人为导向的研究的增长。研究项目。在本申请中，我包括两个以患者为导向的研究项目，这些项目来自我目前的HCV中心资助和使用Virahep-C队列的R 01资助。这两个不同的，特点鲜明的队列包括展示各种研究，将可供未来的学员。我们研究的中心目标是了解控制丙型肝炎病毒感染恢复的免疫因素，无论是自发的还是治疗诱导的。这里提出的新研究的目的是回答在执行资助项目过程中出现的其他问题。 发展规划到目前为止，我指导过的大多数人（12人中的11人）都在追求学术生涯。来自K24的支持将使我从临床和行政责任中解脱出来，并使我能够指导更多的学员，并在UCHSC优秀的科学界探索许多合作机会。 研究环境。本提案中概述的研究将在罗森博士的实验室进行，该实验室位于UCDs研究大楼2的10楼，面积为2，707平方英尺。该实验室包括生物危害层流罩、细胞培养CO2培养箱、倒置和直立显微镜、密封离心机、-70 ° C和液氮冷冻机以及标准PCR设备。该实验室还配备了FACS Aria，Luminex系统和AID自动ELISPOT阅读器。邻近的空间可用于分子生物学和蛋白质表达工作。通过GI分部和科罗拉多艾滋病研究中心（CFAR）可获得各种各样的共享设备，包括流式细胞仪和细胞分选仪（流式细胞仪核心）、高速和超浓缩仪、γ和β计数器、细胞收集器、荧光显微镜、FPLC和自动ABI 377测序仪。 相关性：全世界约有2亿人患有慢性HCV感染，其中很大一部分患者对抗病毒治疗无效。这个K24将提高我指导初级研究人员的能力，并将为这种常见疾病的新型免疫方法提供基础。

DESCRIPTION (provided by applicant): Goals. Immediate and long-term objectives are: 1) to be established as a leader in translational, clinical, and basic research related to liver disease; and 2) to train the next generation of physician-scientists working in gastrointestinal and liver disease. 1 am committed to enhancing the educational training of medical students, residents, fellows, and junior faculty members in research and facilitate the growth of patient-oriented research. Research Project. In this application, 1 have included two patient-oriented research projects that stem from my current HCV Center Grant and an R01 grant using the Virahep-C cohort. These two distinct, well-characterized cohorts are included to demonstrate the variety of research that would be available for future trainees. The central goal of our research is to understand the immune factors that govern recovery from HCV infection, either spontaneously or therapy-induced. The purpose of the new research proposed here is to answer additional questions that have arisen in the course of carrying out the funded projects. Development plan. To date, the majority of individuals I have mentored (11 of 12) have pursued academic careers. Support from this K24 will release me from clinical and administrative responsibilities and allow me to mentor of more trainees and explore the many collaborative opportunities within the outstanding scientific community at UCHSC. Research environment. The studies outlined in this proposal will be performed in Dr. Rosen's lab, 2,707 sq ft on the 10th floor of UCDs Research Complex 2. The laboratory contains biohazard laminar flow hoods, cell culture C02 incubators, inverted and upright microscopes, containment centrifuges, -70oC and liquid nitrogen freezers, and standard PCR equipment. The lab also houses a FACS Aria, Luminex system, and an AID automated ELISPOT reader. Adjacent space is available for molecular biology and protein expression work. A wide variety of shared equipment is available through the Gl Division and the Colorado Center for AIDs Research (CFAR), including flow cytometers and cell sorters (Flow Cytometry Core), high speed and ultracentrifuges, gamma and beta counters, cell harvesters, fluorescent microscope, FPLC, and automated ABI 377 sequencer. RELEVANCE: Approximately 200 million throughout the world have chronic HCV infection, and a large proportion of patients fail to respond to antiviral treatment. This K24 will enhance my ability to mentor junior investigators and will provide the groundwork for novel immunotherapeutic approaches of this common disease.