Core B: Lipidomics Core(Consortium)

项目摘要 PPG中脂质组学共享资源核心（LSR核心）的目的是提供知识和 物理资源（特别是最先进的鞘脂和合成的质谱分析） 研究脂质的分子工具），以增强我们对生物活性脂质在癌症生物学中的作用的理解 目的是发现未来的药物。生物活性脂质的多样性及其相互关联的代谢 产生调节细胞内和细胞间信号传导和功能的通路网络。功能障碍 这些途径有助于癌症进展和转移的病理生物学。这些考虑 已经需要脂质化学和分析的发展。LSR核心是基于 关键人员在脂质化学、分析和代谢方面的独特专业知识。它已经演变成一把钥匙 核心项目赠款（PPG）题为鞘脂在癌症生物学和治疗， 所有参与研究者使用。此外，这一核心已发展成为一个机构性、国家性和 脂质组学和脂质化学生物学新兴领域的国际资源。核心服务包括：1） 提供来自不同生物材料（细胞， 组织和生物流体），主要采用高效液相色谱-串联质谱 质谱（HPLC-MS/MS）技术-核心目前提供了300多个定量分析 在基础代谢组学水平上的不同脂质分子种类; 2）开发和提供合成分子 研究脂质代谢的工具（例如，功能化和荧光神经酰胺，位点特异性放射性 鞘脂，17 C-鞘脂）和用于细胞、体外和体内的多种合成脂质和类似物 研究（例如，靶向细胞器的鞘脂和靶向细胞器的鞘脂代谢抑制剂 3）协助PPG研究者进行实验设计，选择感兴趣的脂质，以及 分析结果的解释。LSR核心对PPG的成功至关重要 在过去的10年里，研究人员通过为他们提供独特的能力和关键的结果， 继续为PPG续约的成功进展做出贡献。

PROJECT SUMMARY The purpose of the Lipidomics Shared Resource Core (LSR Core) in the PPG is to provide intellectual and physical resources (particularly state-of-the-art mass spectrometry analysis of sphingolipids and synthetic molecular tools to study lipids) to enhance our understanding of the role of bioactive lipids in cancer biology with the goal to discover future drugs. The diversity of bioactive lipids and their interconnected metabolism generates a network of pathways regulating intra- and inter-cellular signaling and function. Dysfunctions in these pathways contribute to the pathobiology of cancer progression and metastasis. These considerations have necessitated the development of lipid chemistry and analysis. The LSR Core was created based on unique expertise of the key personnel in lipid chemistry, analysis, and metabolism. It has evolved into a key Core for the program project grant (PPG) entitled Sphingolipids in Cancer Biology and Therapeutics, and was used by all participating investigators. Furthermore, this Core has developed into an institutional, national, and international resource in the emerging fields of lipidomics and lipid chemical biology. Core services include: 1) providing qualitative and quantitative analysis of lipid components from different biological materials (cells, tissue, and biological fluids), primarily employing high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) technology-the Core currently provides quantitative analysis of more than 300 distinct lipid molecular species at a basic metabolomic level; 2) developing and providing synthetic molecular tools to study lipid metabolism (e.g., functionalized and fluorescent ceramides, site-specific radioactive sphingolipids, 17C-sphingolipids) and diversified synthetic lipids and analogs for cellular, in vitro, and in vivo studies (e.g., organelle-targeted sphingolipids and organelle-targeted inhibitors of sphingolipid metabolizing enzymes); and 3) assisting PPG investigators in experimental design, selection of lipids of interest, and interpretation of analytical results. The LSR Core has been instrumental to the success of the PPG investigators over the past 10 years by providing them with unique capabilities and critical results that should continue to contribute for the successful progress of this PPG renewal.