Microscopy and Imaging Analysis Core

项目摘要-M&I核心 显微成像分析(M&I)核心是视觉研究的主要组成部分 加州大学洛杉矶分校的核心，有18个研究实验室预测适度或广泛使用。并购交易 在新实验室和新设备方面都对核心设施进行了改造。这个 核心现在被安置在专门的核心空间，这个空间已经完全翻新。在过去两年中 年，一台新的JEOL JEM 1400plus电子显微镜，一台新的Deltavision OMX超级 购买了分辨率显微镜和新的尼康旋转盘共焦显微镜， 现已全部启动并运行，为用户提供最先进的成像技术。 M&I核心配备了用于：1)样品制备的设施和设备，包括 待研究组织在固定状态下的保存，切片(从厚到超薄)，以及 为活细胞成像准备活组织；2)许多不同形式的成像能力， 从光到超分辨率再到EM；3)图像分析，包括Imaris在内的软件套件。 因此，与各方面的技术支持一起，核心为用户提供便利， 在样品制备、成像和图像获取以及图像方面的援助和培训 分析。 因此，M&I核心促进了由加州大学洛杉矶分校NIH/NEI支持的基础和临床前研究 通过提供无法在中找到的设施、设备和技术技能来访问园区 单独的实验室。

PROJECT SUMMARY – M&I CORE The Microscopy and Imaging Analysis (M&I) core is the major component of the vision research core at UCLA, with 18 research laboratories projecting moderate or extensive usage. The M&I core facilities have been revamped in terms of both new laboratories and new equipment. The core is now housed in dedicated core space that was completely renovated. Over the past two years, a new JEOL JEM 1400plus electron microscope, a new Deltavision OMX super resolution microscope, and a new Nikon spinning disk confocal microscope were purchased, and are now all up and running, providing users with state-of-the-art imaging. The M&I core is equipped with facilities and equipment for: 1) sample preparation, including the preservation of tissue to be studied in a fixed state, sectioning (from thick to ultrathin), and the preparation of live tissues for live-cell imaging; 2) many different forms of imaging capabilities, from light to super resolution to EM; 3) image analysis, with a software suite, including Imaris. Thus, together with technical support in all aspects, the core provides users facilities, assistance, and training in sample preparation, imaging and image acquisition, and image analysis. The M&I core thus facilitates basic and preclinical research supported by NIH/NEI on UCLA campus by providing access to facilities, equipment, and technical skills that cannot be found in individual laboratories.