Antibiotic Resistance Surveillance in Retail Meat and Seafood Samples from South Dakota and North Dakota.

FDA-NARMS合作协议计划（CAP）的目标是建立一个由30个 分布在美国各地的实验室，以确定抗菌药物的流行率， 细菌（沙门氏菌属、弯曲杆菌属、气单胞菌属、弧菌属、大肠杆菌属）的耐药性;大肠杆菌、CPE和 肠球菌）分离自鸡肉、火鸡肉、牛肉、猪肉，罗非鱼， 从杂货店购买的鲑鱼和虾。我们提议的CAP赠款部分将 是为了确定这些从零售商那里分离出来的微生物中抗生素耐药性的普遍性， 来自南达科他州和北达科他州商店的肉类和海鲜样品。一百二十 五年内，每季度将从两个零售肉类和海鲜样本中购买一个。 在南达科他州和北达科他州都有。这些零售肉类和海鲜样本将 交付给动物疾病研究的食品安全微生物学实验室， 南达科他州州立大学诊断实验室。将对所有样本进行检测， 沙门氏菌属、弯曲杆菌属、气单胞菌属、弧菌属、E.大肠杆菌，CPE和肠球菌， 食品安全分析方案。从这些食物样本中分离出的微生物将被运往 FDA-CVM用于分析抗生素耐药性。分离出弯曲杆菌、沙门氏菌和E. 将利用现有的GenomeTrakr对大肠杆菌进行现场测序并上传至FDA数据库 管道这些结果将被汇编并分发回FSM实验室，以便分发给 合作推广服务、消费者和生产者。

The objective of the FDA-NARMS cooperative agreement program (CAP) is to build a network of 30 laboratories distributed throughout the United States to determine the prevalence of antimicrobial resistance among bacteria (Salmonella, Campylobacter, Aeromonas, Vibrio, E. coli, CPE, and enterococci) isolated from samples of chicken, ground turkey, ground beef, ground pork, tilapia, salmon and shrimp purchased from grocery stores. Our proposed portion of this CAP grant would be to determine the prevalence of antibiotic resistance among these microbes isolated from retail meat and seafood samples from South Dakota and North Dakota stores. One hundred and twenty one retail meat and seafood samples will be purchased each quarter for five years from each of two sites in both South Dakota and North Dakota. These retail meat and seafood samples will be delivered to the Food Safety Microbiology (FSM) Laboratory in the Animal Disease Research and Diagnostic Laboratory at South Dakota State University. All samples will be tested for the presence of Salmonella, Campylobacter, Aeromonas, Vibrio, E. coli, CPE and Enterococci using established food safety analysis protocols. The microbes isolated from these food samples will be shipped to FDA-CVM for analysis of antibiotic resistance profiles. Isolates of Campylobacter, Salmonella, and E. coli will be sequenced onsite and uploaded to the FDA database utilizing existing GenomeTrakr pipelines. These results will be compiled and distributed back to the FSM lab for dissemination to the Cooperative Extension Services, Consumers, and Producers.