Biospecimen & Histopathology

核心B是生物标本和组织学核心。该核心负责协助采购 人体组织、细胞分离和培养、细胞系的维持和肺组织的形态学研究 和细胞。形态学组件为PPG项目提供支持，其研究需求为 免疫细胞化学（荧光和过氧化物酶），激光旋转盘共聚焦显微镜， 冷冻切片术，免疫电镜，TUNEL，透射或扫描电镜， 激光捕获显微镜和原位杂交。形态学组件将使用现有的肺 肺科学与健康中心的形态学核心和肺科及生物学 图像处理实验室的激光捕获显微镜。除了提供技术专长和 核心B人员将协助PPG项目 研究人员在程序设计、获得的图像的解释和数据分析中。的范围 来源于对照和IPF肺的成纤维细胞的表型，其通过组织外植体和化学方法获得 将测定四组中每一组的消化并进行比较。此外，表达 将通过每种方法获得的分离的成纤维细胞的表型标记物与 肺内成纤维细胞的标记物，以及IPF肺的成纤维细胞病灶内的标记物。芯会 系统地测试第二抗体和项目中使用的其他常用工具，以优化和 标准化结果。Ingbar博士将指导分配时间的个人实验， 技术人员，并根据需要，将开发定量免疫荧光和原位杂交 使用基于计算机的图像分析系统的技术。超微结构研究将使用其他大学 明尼苏达州电子显微镜实验室否则，所有必要的设备，包括共焦 Core B中提供了带有定量图像分析软件的显微镜。Jose Jesserun博士， 病理学教授，将协助Ingbar博士解释使用免疫染色的人类和 实验动物肺 相关性（参见说明）：

Core B is the Biospecimen and Histopathology Core. This Core is responsible for assistance in procuring human tissues, cell isolation and culture, maintenance of cell lines and morphologic studies on lung tissue and cells. The morphology component provides support for PPG projects with research needs for immunocytochemistry (fluorescence and peroxidase), laser spinning-disc confocal microscopy, cryoultramicrotomy, immunoelectron microscopy, TUNEL, transmission or scanning electron microscopy, laser capture microscopy and in situ hybridization. The morphology component will use the existing Lung Morphology Core in the Center for Lung Science and Health and Pulmonary Division and the Biological Image Processing Laboratory's laser capture microscope. In addition to providing technical expertise and the facilities for carrying out these experiments, the Core B personnel will assist the PPG project investigators in design of procedures, interpretation of the images obtained and data analysis. The range of phenotypes of fibroblasts derived from control and IPF lungs derived by tissue explant and chemical digestion will be determined for each of the four groups and compared. In addition the expression of phenotypic markers of the isolated fibroblasts derived by each method will be compared with expression of markers by fibroblasts within the lung, and for IPF lungs, within the fibroblastic foci. The Core will systematically test secondary antibodies and other common tools used across projects, to optimize and standardize the results. Dr. Ingbar will direct the allocation of time for individual experiments by the technicians and, as needed, will develop quantitative immunofluorescence and in situ hybridization techniques using a computer-based, image analysis system. Ultrastructural studies will use other University of Minnesota electron microscopy labs. Otherwise all necessary equipment, including the confocal microscope with quantitative image analysis software is available within Core B. Dr. Jose Jesserun, Professor of Pathology, will assist Dr. Ingbar in interpretation of studies using immuno-stained human and experimental animal lungs. RELEVANCE (Seeinstructions):