Development of 3D interferometric super-resolution methods for imaging dynamic, multi-component molecular systems, in single cells and in multi-cellular environments

摘要 了解细胞间通讯和集体细胞现象如何塑造细胞功能和细胞命运 决策需要能够跟踪详细的分子过程，因为它们发生在活细胞内， 该细胞保持嵌入在其组织和生物体的天然环境中。虽然巨大的 在单个孤立细胞的成像方面已经取得了进展， 具有高时空分辨率和低至单分子灵敏度的系统呈现出巨大的 光学显微镜的挑战。在这里，我们建议应对这一挑战，利用最近的突破i） 提高从微弱荧光信号中提取高分辨率信息的能力-使用3D 干涉测量; ii）使用优化配置-基于选择性- 计划照明; iii）通过光学不均匀的样本维持/恢复高分辨率信息- 使用自适应光学。我们假设，这三项关键技术的成功综合将创造 新的方法，跨越到以前未知的领域，结合时空分辨率，检测 灵敏度、非侵入性和穿透深度。基于这些想法，我们提出以下两个建议： 具体目标：（1）开发多色体积三维干涉成像，基于可靠的，无伪影 光学重建，用于提高从弱荧光中提取高分辨率信息的能力 （2）实现无创、无背景、长期4D成像，在~ 100 nm近各向同性3D 空间分辨率，在毫秒级采集时间和在大的和高度拥挤的（多）细胞体积。 新技术将显著提高我们询问动态生物过程的能力， 分子细节，在单个分离的细胞以及在完整的复杂的多细胞系统中，因此具有 对生物医学学科产生广泛而直接的影响。

ABSTRACT Understanding how cell-cell communication and collective cell phenomena shape cell function and cell fate decisions requires the ability to follow the detailed molecular processes as they take place inside live cells, with the cell remaining embedded in its natural setting of the tissue and organism of origin. Although tremendous progress has been made in imaging of single isolated cells, the requirements for probing crowded multi-cellular systems with high spatio-temporal resolution and down to single-molecule sensitivity present an enormous challenge for optical microscopy. Here we propose to tackle this challenge, using recent breakthroughs to i) increase the capability of extracting high-resolution information from weak fluorescence signals – using 3D interferometry; ii) image fragile/delicate biological samples using optimized configurations - based on selective- plan illumination; iii) maintain/recover high resolution information through optically inhomogenous samples – using adaptive optics. We hypothesize that successful synthesis of these three key technologies will create new approaches that cross into previously uncharted realms of combined spatio-temporal resolution, detection sensitivity, non-invasiveness and penetration depth. Based on these ideas we propose the following two specific aims: (1) To develop multi-color volumetric 3D interferometric imaging, based on reliable, artifact-free optical reconstructions, for increasing the ability to extract high-resolution information from weak fluorescence signals; (2) To achieve non-invasive, background-free, long-term 4D imaging, at ~100nm near-isotropic 3D spatial resolution, at millisecond acquisition times and over large and highly crowded (multi)cellular volumes. The new techniques will significantly increase our abilities to interrogate dynamic biological processes with molecular detail, in single isolated cells as well as in intact complex multi-cellular systems, thus having widespread and immediate impact across biomedical disciplines.