Bruker Avance III 600 MHz (14T) Wide Bore Spectrometer

描述(由申请人提供)：购买600 MHz宽口径14T成像光谱仪的基本目的是为一个创新的研究小组提供一个独特而强大的新工具，该工具将为推动其研究计划超越目前的限制提供关键。这一新仪器将使新的实验成为可能，并提高一些R01项目的生产率，这些项目研究心脏、肌肉、癌症和衰老生物学中的细胞生理学，重点是完整细胞中的线粒体功能。华盛顿大学在西雅图医学院新建的设施中建立了线粒体和新陈代谢中心，该设施位于南湖联合校区，拥有新招聘的教师。这个小组正在形成一个独特的资源，由Kushmerick博士和Tian博士共同指导，并由医学院麻醉学和疼痛医学系以及医学院和教务长办公室的放射科合作建立，并由它们共同管理。拟议的高场核磁共振光谱仪和显微成像仪将是该中心的主要技术重点，也是医学院和华盛顿大学的独特仪器。“线粒体医学”领域的重要性正在扩大。除了作为细胞代谢能量的主要来源外，线粒体的正常功能和功能障碍在细胞凋亡和对氧化损伤的反应中都起着核心作用。由用户赠款项目开发的创新的方法学和生理学进展揭示了对这些机制的见解和临床应用的线索。例如，随着这台高场光谱仪的加入，我们将能够通过磁共振方法与我们用户实验室中广泛的生物物理、生化、生理和分子生物学方法相结合，获得关于线粒体功能的关键功能数据。该设施将由放射科的MR专家进行维护和质量控制，并由两个部门管理。坚定的用户群体包括资金充足的心脏新陈代谢(田荣博士)、肌肉新陈代谢和能量学(David Marcinek和Martin Kushmerick博士)、老年肌肉研究(Marcinek和Rabinovitch博士)以及小鼠肿瘤生物学和分子成像(Lee栋hoon Lee博士)。此外，我们还有资金雄厚的合作者，他们正在开发核磁共振技术，用于心肌干细胞和再生(Charles Murry博士)、衰老小鼠模型(Peter Rabinovitch博士)、培养的平滑肌细胞(Cecilia Giachelli博士)和代谢组学(David Eaton博士)。拟议的14T仪器将与这些调查人员和大学内的其他人共享。 公共卫生相关性：在这项高端仪器拨款申请中提出的磁共振光谱仪主要用户和次要用户的研究计划包括广泛的项目，重点是促进我们对一系列疾病和疾病的了解和改进诊断方法，包括心力衰竭、衰老、影响肌肉的慢性和系统性疾病以及癌症。拟议的设备是一种非常高分辨率的核磁共振光谱仪和显微成像仪，将用于测试基础科学中的假说，并用于验证可转化为临床应用和诊断程序的方法。由于这台光谱仪的先进性能，购买这台设备将使这些计划朝着新的和令人兴奋的方向前进。

DESCRIPTION (provided by applicant): The fundamental purpose underlying this request for the purchase of a 600 MHz wide bore 14T imaging spectrometer is to provide an innovative research group with a unique and powerful new tool that will provide a key for advancing its research programs beyond current limitations. This new instrument will make new experiments possible and enhance the productivity of a number of R01 projects studying cellular physiology in heart, muscle, cancer and the biology of aging, with emphases on mitochondrial function in intact cells. The University of Washington has established a Mitochondria and Metabolism Center at a newly built facility of the School of Medicine in Seattle, located at the South Lake Union campus with newly recruited faculty. This group is forming a unique resource, co-directed by Drs. Kushmerick and Tian and established by the cooperation of, and jointly administered by, the Departments of Anesthesiology and Pain Medicine and of Radiology in the School of Medicine and the Provost's Office. The proposed high field NMR spectrometer and micro-imager will be a primary technological focus of the Center and a unique instrument in the School of Medicine and at the University of Washington. The field of "mitochondrial medicine" is expanding in importance. Both normal function and dysfunction of mitochondria play a central role in apoptosis and response to oxidative damage in addition to their role as the main source of metabolic energy in the cell. Innovative methodological and physiological advances developed by the users' grant projects have revealed insights into these mechanisms and clues for clinical applications. For example, with the addition of this high field spectrometer, we will be able to obtain critical functional data on mitochondrial function by magnetic resonance methods in conjunction with a wide range of biophysical, biochemical, physiological and molecular biological methods resident in the labs of our users. The facility will be maintained and quality control provided by MR expertise in the Department of Radiology and managed by both departments. The group of committed users includes well-funded projects in cardiac metabolism (Dr. Rong Tian), muscle metabolism and energetics (Drs. David Marcinek and Martin Kushmerick), aged muscle studies (Drs. Marcinek and Rabinovitch) and mouse tumor biology and molecular imaging (Dr. Donghoon Lee). In addition we have well-funded collaborators who are developing NMR techniques for their grants in myocardial stem cell and regeneration (Dr. Charles Murry), in mouse models of aging (Dr. Peter Rabinovitch), in cultured smooth muscle cells (Dr. Cecilia Giachelli) and in metabolomics (Dr. David Eaton). The proposed 14T instrument will be shared with those investigators and others within the University. PUBLIC HEALTH RELEVANCE: The research programs of the major and minor users of the magnetic resonance spectrometer proposed in this high-end instrument grant application encompass a wide scope of projects focused on furthering our understanding of and advancing diagnostic approaches for a range of disorders and diseases, including heart failure, aging, chronic and systemic diseases affecting muscle, and cancer. The equipment proposed is a very high resolution NMR spectrometer and microimager that will be used for testing hypotheses in basic science and serve to validate methods that can be translated into clinical applications and diagnostic procedures. The purchase of this equipment will allow these programs to advance in new and exciting directions, due to the advanced capabilities of this spectrometer.