Leica SPX5 2-photon laser-scanning confocal microscope system

描述(由申请人提供)：本申请申请为密歇根大学安娜堡校区购买徕卡SP5X双光子激光扫描共聚焦显微镜系统。目前，密歇根大学还没有共享使用的双光子共聚焦显微镜系统。该系统由徕卡SP5X倒置显微镜组成，配置为接受相干超II泰坦蓝宝石红外激光进行双光子成像。该系统是一种较新形式的共聚焦显微镜，允许对感兴趣的活体系统、细胞、分子和离子分布进行实时重复成像，同时避免与传统共聚焦显微镜相关的光漂白和光毒性问题。徕卡系统之所以被选中，是因为它在发射光谱选择方面的技术进步，易于操作的软件，以及来自当地服务提供商的快速技术援助。该系统将安装在显微镜和图像分析实验室(MIL)内，这是一个专业管理的大学成像核心，网址为www.milImaging.com。我们预计，在密歇根大学校区，多达70名由NIH赞助的主要调查人员、他们的合作调查人员、学生以及研究社区中的其他人都有对NIH进步具有技术兴趣的用途。将受益于该仪器的项目包括树突状和轴突发育的机制、细胞的分泌过程、染色体组织、听力损失和肿瘤转移过程等。日常维护、技术监督和指导将由MIL工作人员进行。正在进行的维护将通过MIL使用的标准充值系统进行。长期连贯性由多边基金指定的储备基金提供支持。

DESCRIPTION (provided by applicant): This application requests funds to purchase a Leica SP5X 2-photon laser-scanning confocal microscope system for the University of Michigan campus in Ann Arbor, Michigan. Currently there is no 2-photon confocal microscope system for shared use at the University of Michigan. The system consists of a Leica SP5X inverted microscope configured to accept a Coherent ultra II Titan Sapphire IR laser for 2-photon imaging. This system is a newer form of confocal microscopy which allows real-time repeated imaging of living organism systems, cells, molecules, and ion distribution of interest while avoiding the photobleaching and phototoxicity problems associated with conventional confocal microscopes. The Leica system was selected because of its technological advancements with emission spectral selection, ease of operating software, and fast technical assistance from local service providers. The system will be housed within the Microscopy & Image Analysis Laboratory (MIL), a professionally managed University- wide imaging core, (www.milimaging.com). We anticipate that up to 70 NIH sponsored primary investigators, their co-investigators, and students as well as others in the research community on the University of Michigan campus have uses which are of technical interest to NIH advancements. Projects that will benefit from the instrumentation include mechanisms for dendritic and axonal development, secretory processes of cells, chromosomal organization, hearing loss, and tumor metastasis processes to name a few. Day-by-day maintenance, technical supervision, and instruction will be carried out by members of the MIL staff. Ongoing maintenance will be covered through a standard recharge system used in the MIL. Long-term continuity is supported by a designated reserve fund for the MIL.