Microscopy and Imaging Analysis Core

项目概要- M&I核心 显微镜和图像分析（M&I）核心是视觉研究的主要组成部分 核心在加州大学洛杉矶分校，与18个研究实验室预测中度或广泛使用。M&I 核心设施在新实验室和新设备方面得到了改造。的 核心现在设在专门的核心空间，完全翻新。过去两 年，新的JEOL JEM 1400 plus电子显微镜，新的Deltavision OMX超级 分辨率显微镜和新的Nikon旋转盘共聚焦显微镜， 现在已经全部投入使用，为用户提供最先进的成像技术。 M&I核心配备了以下设施和设备：1）样品制备，包括 将待研究的组织保存在固定状态下，切片（从厚到厚）， 用于活细胞成像的活组织的制备; 2）许多不同形式的成像能力， 从光到超分辨率再到EM; 3）图像分析，包括Imaris软件套件。 因此，该核心加上各方面的技术支持，为用户提供便利， 在样品制备、成像和图像采集以及图像处理方面提供协助和培训。 分析. 因此，M&I核心促进了NIH/NEI支持的UCLA基础和临床前研究 校园提供访问设施，设备和技术技能，无法找到在 个别实验室。

PROJECT SUMMARY – M&I CORE The Microscopy and Imaging Analysis (M&I) core is the major component of the vision research core at UCLA, with 18 research laboratories projecting moderate or extensive usage. The M&I core facilities have been revamped in terms of both new laboratories and new equipment. The core is now housed in dedicated core space that was completely renovated. Over the past two years, a new JEOL JEM 1400plus electron microscope, a new Deltavision OMX super resolution microscope, and a new Nikon spinning disk confocal microscope were purchased, and are now all up and running, providing users with state-of-the-art imaging. The M&I core is equipped with facilities and equipment for: 1) sample preparation, including the preservation of tissue to be studied in a fixed state, sectioning (from thick to ultrathin), and the preparation of live tissues for live-cell imaging; 2) many different forms of imaging capabilities, from light to super resolution to EM; 3) image analysis, with a software suite, including Imaris. Thus, together with technical support in all aspects, the core provides users facilities, assistance, and training in sample preparation, imaging and image acquisition, and image analysis. The M&I core thus facilitates basic and preclinical research supported by NIH/NEI on UCLA campus by providing access to facilities, equipment, and technical skills that cannot be found in individual laboratories.