Defining and targeting mechanisms of pancreas cancer pathogenesis

项目摘要 马丁·惠特尔博士是弗雷德·哈钦森研究所苏尼尔·辛戈拉尼博士实验室的资深科学家。 癌症研究中心。惠特尔博士的训练为他在分子科学领域奠定了坚实的基础 生物学、癌细胞信号和小鼠肿瘤模型。这一研究专家奖将支持Dr。 惠特尔在整个欣戈拉尼实验室研究计划的核心研究项目上所做的努力，包括 以下三个关键项目：复杂的胰腺癌微环境及其对疾病的贡献 PDA发病机制与耐药性、免疫反应及有效药物的研究进展 免疫疗法；以及胰腺癌转移开关的分子基础。除了 惠特尔博士为这些项目中的每一个都进行了重要的实验，他通过以下方式进一步支持Hingorani实验室 对新的技术人员和博士后研究员进行基础和高级实验室技术方面的培训 对Hingorani研究计划至关重要，包括基因工程、小鼠模型开发和 特性、畜牧学、外科手术和尸检、原代组织培养、组织化学 免疫组织学、基因分型、聚合酶链式反应、蛋白印迹、流式细胞仪、荧光显微镜、基因 芯片、分子克隆、酶联免疫吸附试验、质谱学等。

Project Summary Dr. Martin Whittle is a senior scientist working in the laboratory of Dr. Sunil Hingorani at the Fred Hutchinson Cancer Research Center. Dr. Whittle’s training has provided him a strong foundation in the fields of molecular biology, cancer cell signaling, and tumor modeling in mice. This Research Specialist award will support Dr. Whittle’s effort on research projects central to the overall Hingorani Laboratory research program including the following three key projects: the complex pancreas cancer microenvironment and its contributions to disease pathogenesis and drug resistance; the immune reaction in PDA and development of effective immunotherapies; and the molecular underpinnings of the metastatic switch in pancreas cancer. Aside from performing vital experiments for each of these projects, Dr. Whittle further supports the Hingorani lab by training new technicians and postdoctoral fellows in fundamental and advanced laboratory techniques that are critical to the Hingorani research program, including genetic engineering, mouse model development and characterization, animal husbandry, surgical procedures and necropsies, primary tissue culture, histochemistry and immunohistology, genotyping PCR, qRT-PCR, western blotting, FACS, fluorescence microscopy, gene array, molecular cloning, ELISA, mass spectrometry and others.