Maintenance of the SPF Breeding Colonies at Yerkes National Primate Research Center: Husbandry and Management Core

项目摘要/摘要 畜牧业与管理核心 耶克斯国家灵长类动物研究中心(YNPRC)有一段非常成功的历史 培育印度血统的恒河猴，用于艾滋病毒/艾滋病研究。这一成功可以部分归因于 自2002年以来，U24一直为这个殖民地的管理和照顾提供支持。U24支持允许 YNPRC野外站恒河猴繁殖群体建立系谱，向全群过渡 无特定病原体(SPF)状态，引入主要组织相容性(MHC)分析，并验证 B型疱疹病毒(猕猴疱疹病毒1)快速筛查方案。在过去的4年里，重要的是， 由美国国立卫生研究院资助的需要更多SPF猕猴的艾滋病毒/艾滋病相关项目的数量已经 显著增加。还预计这一趋势将在下一次U42资金中继续增加 期间，如总体部分中所述。因此，畜牧管理的中心目标是 当前U42应用的核心是利用U42提供的资源和U42的专业知识 YNPRC动物资源司，以最大限度地生产印度原产恒河猴，并促进 将这些动物分配给NIH资助的艾滋病毒/艾滋病研究。作为目标1的一部分，畜牧业和 管理核心将继续维护SPF状态和遗传健康以及整体体检 和社会健康，以培育出具有所需MHC基因型别的高质量动物 艾滋病毒/艾滋病研究。作为最大化畜牧业生产的目标2的一部分，畜牧业和管理核心 将继续采用他们行之有效的管理做法，保持社会稳定的复合体，并运行- 类型繁育群体，但也将实施新的雄性繁殖者社会化计划，以提高 多个雄性群体融入已建立的基于多母系化合物的繁育群体的比率。 最后，作为目标3的一部分，畜牧业和管理核心将简化动物分配 通过实施动态人口建模程序和对Yerkes的增强进行艾滋病毒/艾滋病研究 基于Web的电子动物搜索工具。这些目标的成功将确保SPF菌落在 YNPRC将继续增长，并提供可靠的健康和基因特征良好的恒河猴供应 用于艾滋病毒/艾滋病调查的猕猴。

Project Summary/Abstract Husbandry and Management Core The Yerkes National Primate Research Center (YNPRC) has an exceptionally successful history of breeding rhesus macaques of Indian-origin for HIV/AIDS research. This success can be attributed, in part, to continuous U24 support for the management and care of this colony since 2002. U24 support has allowed the rhesus macaque breeding colony at the YNPRC Field Station to establish a colony pedigree, to transition to full specific pathogen free (SPF) status, to introduce major histocompatibility (MHC) profiling, and to validate a rapid in-house Herpes B virus (Macacine herpesvirus 1) screening protocol. Over the past 4 years, importantly, the number of individual NIH-funded HIV/AIDS-related projects requiring more SPF rhesus monkeys has increased significantly. It is also expected that this trend will continue to increase over the next U42 funding period as described in the Overall Section. Thus, the central objective of the Husbandry and Management Core for the current U42 application is to leverage the resources provided by the U42 and the expertise of the Division of Animal Resources at YNPRC to maximize production of Indian-origin rhesus monkeys and facilitate allocation of these animals for NIH-funded HIV/AIDS research. As part of Aim 1, the Husbandry and Management Core will continue to maintain the SPF status and genetic health as well as the overall physical and social health of the Colony in order to produce high quality animals with desired MHC genotypes for HIV/AIDS research. As part of Aim 2 to maximize animal production, the Husbandry and Management Core will continue to employ their proven management practices of maintaining socially-stable compound and run- type breeding groups, but will also implement a new male breeder socialization program to improve the integration rate of multi-male cohorts into established multi-matrilineal compound-based breeding groups. Finally, as part of Aim 3, the Husbandry and Management Core will streamline animal assignments for HIV/AIDS research by implementing a dynamic population modeling program and enhancements to a Yerkes web-based Electronic Animal Search Tool. The success of these aims will ensure that the SPF colony at YNPRC will continue to grow and provide a reliable supply of healthy and genetically well-characterized rhesus macaques for HIV/AIDS investigations.