Resource Section - Core 001

项目总结 几十年来，小鼠一直是研究人类疾病的主要动物模型，提供了 探索直接在人类身上研究难以解决的实验问题的机会。作为一名 结果，获得了针对各种临床情况的关键见解。受到科技进步的刺激 系统地敲除所有编码蛋白质的基因的技术和国际努力 在基因组方面，转基因小鼠的数量在过去20年里迅速扩大。这个 最初，维护和分发突变小鼠的责任由个人承担 调查人员，这在成本、劳动力和空间方面都是巨大的负担。1999年，美国国立卫生研究院 认识到需要一种资源来保护对突变小鼠品系的投资，并确保开放 接触到所有生物医学研究人员并建立了突变小鼠区域资源中心 (MMRRC)。自1999年以来，MMRRC-UNC的中心目标一直是与 MMRRC财团合作制定和维持高标准的进口、再衍生、 超低温保存，并将突变小鼠品系分配给生物医学研究人员。MMRRC- 北卡罗来纳州将在下一个项目期继续为财团的目标作出贡献，具体工作是 执行以下具体目标，以继续发挥其作为冷冻档案的成功作用 配送中心，并纳入研究目标，与 资源：1)精简和改进操作程序，以增加进口、分销和 小鼠品系的冷冻档案。2)建立、维护和分发一个全面的冷冻档案 协作交叉(CC)资源。3)开发和传播小鼠遗传计算工具 QC和基因组研究，这将提高表型的重复性。4)调查互动情况 遗传学和环境对实验变异性和重复性的影响。 好了！

PROJECT SUMMARY For decades, the mouse has been the premier animal model for studies of human disease, providing the opportunity to explore experimental questions that are intractable for direct study in humans. As a result, key insights have been obtained for a variety of clinical conditions. Spurred by advances in technology and international efforts to systematically knock out all of the protein coding genes in the genome, the number of genetically engineered mice has expanded rapidly in the past 20 years. The responsibility for maintenance and distribution of mutant mice was initially placed on individual investigators, which was a tremendous burden in terms of cost, labor and space. In 1999, the NIH recognized the need for a resource to protect the investment in mutant mouse strains and ensure open access to all biomedical researchers and established the Mutant Mouse Regional Resource Centers (MMRRC). Since 1999, the central goal of the MMRRC-UNC has been to work collectively with MMRRC consortium partners to develop and maintain high standards for importation, rederivation, cryopreservation, and distribution of mutant mouse strains to biomedical investigators. The MMRRC- UNC will continue to contribute to the goals of the Consortium in the next project period by implementing the following Specific Aims devoted to continuing its successful role as a cryoarchive and distribution center and incorporating research goals that synergize with and extend the value of the resource: 1) Streamline and improve operating procedures to increase importation, distribution, and cryoarchive of mouse strains. 2) Establish, maintain, and distribute a comprehensive cryoarchive for the Collaborative Cross (CC) resource. 3) Develop and disseminate computational tools for mouse genetic QC and genomics research, which will enhance phenotypic reproducibility. 4) Investigate the interaction of genetics and environment on experimental variability and reproducibility. !